中国畜牧兽医2011，Vol.38Issue(2)：68-72,5.
鸡小肠上皮细胞的分离培养与鉴定
Culture of Chicken Intestine Epithelial Cells in vitro and its Characterization
李艳 1彭春燕 1梁榕旺 2赵国琦 1金晓君1
作者信息
- 1. 扬州大学动物科学与技术学院,江苏扬州,225009
- 2. 广东博罗县畜牧兽医局,广东博罗,516100
- 折叠
摘要
Abstract
In this study,we selected tissue culture method to isolation chicken intestinal epithelial cells (IEC), using curetrage, phase contrast digest and adherence methods to purify cells, with 0.05 ％ Trypsin to digest and passage, immunocytochemical method to identify intestinal epithelial cells.The results showed that hadro-activity intestinal epithelial cells could be dissociated by tissue culture method, and obtain purified intestine epithelium.Morphology and immunocytochemical method showed that intestinal epithelial cells surface antigen demonstrate masculine, and purified intestine epithelium could be passaged in vitro.关键词
鸡胚/小肠上皮细胞/体外培养/鉴定分类
农业科技引用本文复制引用
李艳,彭春燕,梁榕旺,赵国琦,金晓君..鸡小肠上皮细胞的分离培养与鉴定[J].中国畜牧兽医,2011,38(2):68-72,5.
