疑难病杂志2011，Vol.10Issue(5)：366-368,封3,4.DOI:10.3969/j.issn.1671-6450.2011.05.017
血管紧张素-(1-7)对血管紧张素II诱导的肾小管上皮细胞E-cadherin、α-SMA表达的影响
Influence of angiotensin-(1-7) on expression of E-cadherin and α-SMA induced by angiotensin II in rat's tubular epithelial cells
摘要
Abstract
Objective To investigate the influence of angiotensin-( 1-7 )[ Ang-( 1-7 )] on expression of E-cadherin and α-SMA induced by angiotension II( Ang II ) in rats tubular epithelial cells. Methods The NRK52E were maintained and sub cultured, and then treated with Ang-( 1-7 ) ( 10 mol/L ) and Ang II( 10 mol/L ) for 24, 48, 72,96 hours, the protein expressions of E-cadherin and α-SMA were detect by immunocytochemistry method; The mRNA expression of E-cadherin and α-SMA was detected by real time PCR( RT-PCR ). Results Treated with ang I after 96 h, the protein and mRNA expression of E-cadherin decreased significantly( P ＜ 0. 05 ), but the protein and mRNA expression of α-SMA increased significantly ( P ＜ 0.05 ); treated with Ang II and Ang-( 1-7 ),the protein and mRNA expression of E-cadherin increased significantly ( P ＜ 0.05 ), but the protein and mRNA expression of α-SMA decreased significantly( P ＜0.05 ). Conclusion Ang-( 1-7 ) can inhibit the expression of α-SMA induced by Ang II and up regulate the expression of E-cadherin.关键词
血管紧张素-(1-7)/血管紧张素Ⅱ/E-cadherin/α-SMA/大鼠引用本文复制引用
袁波,贺红焰,王明勇,刘建..血管紧张素-(1-7)对血管紧张素II诱导的肾小管上皮细胞E-cadherin、α-SMA表达的影响[J].疑难病杂志,2011,10(5):366-368,封3,4.基金项目
国家自然科学基金(No.30771008) （No.30771008）
