医药导报2011，Vol.30Issue(5)：561-562,2.DOI:10.3870/yydb.2011.05.002
鳖甲抗肝纤维化活性多肽的固相合成
Solid-Phase Synthesis of Active Anti-hepatic Fibrosis Peptides of Carapax Trionycis
胡春玲 1唐尹萍 1施婧妮 1刘焱文1
作者信息
- 1. 湖北中医药大学中药资源与中药复方省部共建教育部重点实验室,武汉,430065
- 折叠
摘要
Abstract
Objective To study the solid-phase synthesis of the active anti-hepatic fibrosis peptides of Carapax Trionycis, and provide the theoretical basis for developing the medicines against hepatic fibrosis. Methods Solid-phase synthesis was carried out with resin Wang as a carrier, Fmoc as an amino acids protector. After condensed with the mix reagents of TBTV/NMM, deprotected with 20％ priperdine, the crude produts of synthetic peptide were cut down from the wang resin by the cleared reagents TFA/TLS/H2O. Results The purity of the target peptides was over 98％ by RP-HPLC analysis, the molecular weight of which was identical with the theoretical value by mass-spectrum identification. Conclusion This synthesis method is mild, safety, efficient and easy to operate, which can be applied to the large scale synthesis of objective peptides.关键词
鳖甲/多肽/固相合成分类
医药卫生引用本文复制引用
胡春玲,唐尹萍,施婧妮,刘焱文..鳖甲抗肝纤维化活性多肽的固相合成[J].医药导报,2011,30(5):561-562,2.
