山东农业科学Issue(2)：1-6,6.
水稻条纹叶枯病毒RNA干扰载体的构建及遗传转化
Construction and Genetic Transformation of RNA Interference Vectors for Rice Stripe Virus
摘要
Abstract
Twelve pairs of primers were designed according to the known sequences of rice stripe virus,and 5 sequences were obtained by RT- PCR. By BLAST analysis, no similar sequences in rice genome were found matching these five sequences, so they could be used as the interference sequences. A modified pUC19 vector inserted a fragment as intron with a pair of isocaudarners on each side was used as the interference vector. After inserting the interference sequences into the interference vector separately, the complete interference fragments were integrated into the modified binary vector pCAMBIA1300 using ubiquifin as promoter and bar gene as selectable marker. The transgenic rice seedlings were obtained by Agrobacterium -mediated transformation to rice variety Shengdao 13. This provided candidate materials for breeding new rice varieties resistant to rice stripe virus.关键词
水稻条纹叶枯病毒/RNA干扰载体/Ubi启动子/bar基因/抗病水稻品种Key words
Rice stripe virus/ RNA interference vector/ Ubiquitin promoter/ bar gene/ Rice variety with disease resistance分类
生物科学引用本文复制引用
李海青,柳絮,李臻,姚方印,刘炜..水稻条纹叶枯病毒RNA干扰载体的构建及遗传转化[J].山东农业科学,2011,(2):1-6,6.基金项目
国家转基因植物新品种培育重大专项(编号:2009ZX08001-010B)资助. （编号:2009ZX08001-010B）
