摘要
Abstract
Purpose To compare the animal models between hypoxic pulmonary hypertension and pulmonary hypertension due to chronic bronchitis and emphysema, and to provide a better experimental model for the study of formation mechanism of pulmonary hypertension in chronic obstructive pulmonary disease.Methods 24 male SD rats were randomly divided into 3 groups: 10％ of the hypoxic group ( group A, 8 rats ), pulmonary hypertension due to chronic bronchitis and emphysema group ( group B, 8 rats ), normal control group ( group C , 8 rats ). The rats in group of hypoxic pulmonary hypertension were exposed to inhale 10％ oxygen for 2 weeks,and another rats in the group B were intratracheally instilled of lipopolysaccharide ( LPS ) and daily exposed to cigarette smog and chronic hypoxia ( FiO2 =0. 18 )were exposed to produce the animal models. The hlood gas analysis, pulmonary hemodynamic changes and the morphological changes of airways and pulmonary vascular structure were in bronhoalveocar lavage fluids( BALF )were measured. HE staining of lung tissue or triple staining was used to observe the airway inflammation and pulmonary vascular remodeling in pathological changes. Results ( 1 ) Compared with group C, the right ventricular systolic pressure ( RVSP ), mean pulmonary arterial pressure ( mPAP ), right ventricle and left ventricle plus septum weight ratio ( RV/LV + S ), the muscularization of intra-alceolar arteries and the cross-sectional medial vascular wall area of pulmonary arteries which of group A and B were higher than that of group C ( P ＜ 0. 05 ). ( 2 ) Leukocyte count and category of BALF display that there was no difference of WBC in group A and group C, but the leukocytes and neutrophils were increased in group B ( P ＜ 0. 05 ). ( 3 )Airway inflammation suggest that : The main pathological manifestations of group A is that epithelial cell necrosis and goblet cell hyperplasia mainly mucus, but inflammatory cell infiltration was not obvious. To the contrary, airway inflammatory changes of group B consist with that of chronic bronchitis, emphysema, and there is a variety of inflammatory cells in airway wall especially lymphocytes. Conclusions The changes of group B reflect not only the airway inflammation in chronic bronchitis and emphysema but also the characteristic of pulmonary vascular remodeling, which is more suitable for the research of formation mechanism of pulmonary hypertension in chronic obstructive pulmonary disease.关键词
肺动脉高压/动物模型/低氧/慢性阻塞性肺疾病分类
医药卫生
