中国当代儿科杂志2011，Vol.13Issue(2)：145-148,4.
L-精氨酸对宫内发育迟缓大鼠肾脏Pax2表达的影响
Effect of L-arginine on Pax2 expression in the kidneys of pup rats with intrauterine growth retardation
盖建芳 1谢宗德 2李雯 2李素萍 2胡劲涛2
作者信息
- 1. 山西省儿童医院,030013.
- 2. 中南大学湘雅二医院新生儿科,湖南,长沙,410011
- 折叠
摘要
Abstract
Objective To study the effect of L-arginine (L-Arg) on Pax2 expression in the kidneys of pup rats with intrauterine growth retardation (IUGR). Methods Pregnant rats were randomly assigned into three groups :normal, IUGR and L-Arg treated IUGR. The rats in the normal group were fed with ordinary forage (21％ protein) during pregnancy. Those in the other two groups were fed with low diet forage ( 10％ protein) during pregnancy. The L-Arg treated group was given drinking water containing L-Arg ( 200 rag/kg) daily during 21 days of lactation. Pax2 expression in renal tissues was measured with immunohistochemical staining and Western blot in pup rats of 7 days, 21 days, 2 months and 3 months old. Results The immunohistochemical staining showed that Pax2 was not expressed in the pup rats from the normal group at any time point. Pax2 positive ceils were found in renal glomerulus and kidney tubules of 2-months- and 3-months-old rats from the IUGR and L-Arg treated groups. And Pax2 expression in 3-months-old rats was significantly higher than that in 2-months-old rats (P ＜ 0.05 ). L-Arg treatment decreased significantly the Pax2 expression in 2-months- and 3-months-old rats when compared with the untreated IUGR group ( P ＜ 0.05 ). Western blot showed that Pax2 protein was not expressed in 7-days- and 21-days-old pup rats from three groups. Pax2 protein expression in 2-months- and 3-months-old pup rats from the IUGR and L-Arg treated groups increased significantly compared with normal controls. Pax2 protein expression in the pup rats from the L-Arg treated group was significantly lower than that in the untreated IUGR pup rats ( P ＜ 0. 0l ). Conclusions Pax2 is expressed in the kidneys of IUGR rats during adulthood. L-Arg treatment can decrease the expression of Pax2.关键词
宫内发育迟缓/Pax2/肾脏/L-精氨酸/大鼠分类
医药卫生引用本文复制引用
盖建芳,谢宗德,李雯,李素萍,胡劲涛..L-精氨酸对宫内发育迟缓大鼠肾脏Pax2表达的影响[J].中国当代儿科杂志,2011,13(2):145-148,4.
