医药导报2011，Vol.30Issue(5)：581-584,4.DOI:10.3870/yydb.2011.05.008
高效液相色谱法测定人红细胞硫鸟嘌呤核苷酸的浓度
Quantitation Determination of 6-thioguanine Nucleotides in Red Blood Cells by HPLC
摘要
Abstract
Objective To establish a HPLC method for determination of 6-thioguanine nucleotides( 6-TGNs )in red blood cells( RBC ).Methods The RBC samples were deproteinated by 70％ perchloric acid.6-TGNs were hydrolyzed to 6-thioguanine( 6-TG )by heating for 45 min at 100 ℃.Separation was carried out on Hypersil GOLD C18 column( 4.6 mm ×250 mm, 5 μm )with UV detection at 342 nm.The mobile phase consisted of 20 mmol· L-1 KH2 PO4( pH 3.3 )-acetonitrile( 95:5, V/V)with flow rate of 1.0 mL · min-1.Results The linear relationship was occurred over the range of 8.09-809.89 pmol · ( 8×l08 ) -1 RBC for 6-TG ( r = 0.997 8 )and the limit of quantitation was 8.09 pmol · ( 8×l08 ) -1 RBC.The intraand inter-assay coefficients of variation were less than 7.71％ and 6.64％, respectively.The mean analytical recoveries were 98.07％ ～108.08％ , and the mean extraction recoveries were all more than 60％.Conclusion This method is simple,rapid, sensitive and specific, which could be used for quantitation of 6-TGNs in RBC from patients under AZA therapy.关键词
硫唑嘌呤/硫鸟嘌呤核苷酸/6-硫鸟嘌呤/色谱法/高效液相分类
医药卫生引用本文复制引用
师少军,刘亚妮,吴健鸿,曾繁典..高效液相色谱法测定人红细胞硫鸟嘌呤核苷酸的浓度[J].医药导报,2011,30(5):581-584,4.基金项目
湖北省自然科学基金资助项目(基金编号:2009CDB380) （基金编号:2009CDB380）
