肿瘤预防与治疗2011，Vol.24Issue(3)：137-140,4.DOI:10.3969/j.issn.1674-0904.2011.03.001
大肠癌裸小鼠原位移植肝转移模型的建立与比较
Establishment and Comparison of Liver Metastasis Models of Human Colorectal Carcinoma in Nude Mice by Orthotopic Implantation
摘要
Abstract
Objective : To establish a feasible and stahle liver metastasis model of human colorectal carcinoma m nude mice by orthotopic implantation. Methods : Human colonic carcinoma cell line of HCT-8 or HCT-116 was injected into the cecal subserosa or rectal submucosa of BALB/e nude mice, or orthotopic implantation with fresh tumor tissue was performed to create live metastasis. Efficiency of primary tumor formation,occurrence of live metastasis and life duration of tumor bearing mice was compared. Results : The rate of primary tumor formation was 83％ ( 10/12 ) with surgical transplantation of HCT-8 tumor tissue into the cecal subserosa. which was higher than that of injection of tumor cells into the cecal subserosa ( 16％ , 2/12 ) and rectal suhmucoas ( 75％ . 9/12 ). Survival duration of tumor bearing mice was 134 days ( 102 days ～174 days ), 80 days ( 67 days ～93 days ), and 72 days( 58 days ～86 days ) respectively for them. No occurrence of live metastasis was observed with HCT-8 cell lines in all these mice. However. with transplan tation of HCT- 116 tumor tissue into the cecal subserosa, a higher rate of tumorigenecity ( 90％ ,18/20 ) was achieved. Live metastasis emerged 6 weeks after implantation in 25％ ( 1/4 ) of mice, and the rate increased to 75％ ( 3/4 ) two weeks later, with some of the mice ( 25％ , 1/4 ) simultaneously accompanied by lung metastasis. Conclusion : Surgical embedding of fresh HCT- 116 tumor tissue into the cecal subserosa is an ideal way to create liver metastasis model by orthotopic implantation, with stahle tumorigenecity and high spread incidence.关键词
BALB/c裸小鼠/大肠癌/肝转移/动物模型分类
医药卫生引用本文复制引用
郑阳春,陈廷清,张文彬,李吉满,杨敏,燕锦..大肠癌裸小鼠原位移植肝转移模型的建立与比较[J].肿瘤预防与治疗,2011,24(3):137-140,4.基金项目
国家自然科学基金资助课题(NO.30700802) （NO.30700802）
