肿瘤预防与治疗2011，Vol.24Issue(3)：145-148,4.DOI:10.3969/j.issn.1674-0904.2011.03.003
WT1反义寡核苷酸对人卵巢癌裸鼠皮下移植瘤形成的抑制作用
Inhibitory Effect of Antisense Oligodeoxynucleotide Targeting WT1 on the Human Ovarian Carcinoma Transplanted Subcutaneously in Nude Mice
摘要
Abstract
Objective : To study the inhibitory effect of WT1 antisense oligodeoxynucleotides( ASODN ) transfection on the human ovarian epithelial cancer transplanted subcutaneously in nude mice. Methods : Ovarian carcmoma cell SKOV3 lines were treated with WTI-ASODN. and then transplanted subcutaneously in nude mice. The changes of tumor volume were observed and the tumor growth inhihitory rate was calculated. Results: The tumorigenic ability of ovarian carcinoma cell lines SKOV3 transfected by WT1 ASODN was reduced. the time that tumor could be first detected was prolonged and the maximum tumor growth inhibitory rate was increased. Conclusion: WT1 antisense phosphorothioate oligonucleotides can inhibit the tumorigenesis of ovarian epithelial carcinoma cell lines in nude mice. It maybe a valuable gene therapy for the ovarian epithelial carcinoma.关键词
WT1基因/上皮性卵巢癌/反义寡核苷酸/裸鼠皮下移植瘤分类
医药卫生引用本文复制引用
霍晓溪,任丽娜,肖英,卢翠敏,孙莹,尚丽新..WT1反义寡核苷酸对人卵巢癌裸鼠皮下移植瘤形成的抑制作用[J].肿瘤预防与治疗,2011,24(3):145-148,4.基金项目
中国博士后科学基金资助项目(No.20100471759) （No.20100471759）
