医药导报2011，Vol.30Issue(5)：635-637,3.DOI:10.3870/yydb.2011.05.029
大孔树脂纯化穿山龙总皂苷的工艺优化
Optimization of Technology of the Purification of Saponins from Dioscorea Nipponica by Macroporous Resin
摘要
Abstract
Objective To research the isolate and purification crafl of Con-saponin of Dioscoreae Nipponicae, and provided the theoretical foundation and reference value to producing the beverage in industrialization. Methods The content of Protodioscin was chosen as index to the comprehensive assessment. To optimize the variety of macro-reticular resin, different loading quantities, different sample concentration, eluent concentration , elution volume. Results The optimized craft was: D101 macro-reticular resin, sample concentration 0.5 g · mL-1 , loading quantities 2: 1( macro- reticular resin : crude drug of Dioscoreae Nipponicae ), eluent concentration 50％ alcohol, elution volume 14BV. Conclusion This craft is simple and feasible with good reproducible and its active ingredients is more than 60％ , and it can provide the basis for the production.关键词
穿山龙/大孔吸附树脂/原薯蓣皂苷/纯化分类
医药卫生引用本文复制引用
刘颖,刘树民,于栋华,刘磊,杨超..大孔树脂纯化穿山龙总皂苷的工艺优化[J].医药导报,2011,30(5):635-637,3.基金项目
黑龙江省青年科学基金项目(基金编号:QC2009C46) （基金编号:QC2009C46）
