针灸临床杂志2011，Vol.27Issue(2)：63-65,3.
头穴丛刺结合环境刺激对缺氧缺血性脑损伤新生大鼠海马MAP-2和p38表达影响的实验研究
An Experimental Study on the Effects of Cluster Needling of Scalp Point Therapy Combined with Environmental Stimulation on Microtubuleassociated Protein-2 and Synaptophysin of Neonatal Rats with Hypoxic-ischemic Brain Injury
摘要
Abstract
Objective :To discuss the effects of cluster needling of scalp point therapy combined with environmental stimulation on microtubule - associated protein - 2 and synaptophysin of neonatal rats with hypoxic - ischemic brain damage. Methods:7 days Wistor rats by ligation of the left common carotid artery were exposed to 8％ of the nitrogen - oxygen mixture for two hours to make hypoxic ischemic encephalopathy (HIE) model.They are randomly divided into 5 groups:16 rats in sham operation group(A), 16 rats in operation group(B),16 rats in cluster needling of scalp point therapy group( C), 16 rats in environmental stimulation group( D), 16 rats in cluster needling of scalp point therapy combined with environmental stimulation group. On the 14th and 28th day, the immunohistochemical stain and RT -PCR were respectively used to detect the expression of microtubule -associated protein -2 and Hippocampal synaptophysin. Results:On the 14th and 28th day, the immunohistochemical analysis of the treatment group showed the microtubule - associated protein - 2 and P38 in Hippocampus of C, D, E groups increased as compared with that of A, B groups. On the 14th and 28th day,E group had the significant difference with C, D groups by comparison( P ＜ 0.01 ). Conclusion:The cluster needling of scalp point therapy combined with environmental stimulation can improve microtubule - associated protein -2 and the positive expression of the P38 in Hippocampus of the HIBD rats.关键词
缺氧缺血性脑损伤/头穴丛刺/环境刺激分类
医药卫生引用本文复制引用
刘波,唐强,邢艳丽,白震民,颜培宇,关莹,孔妍..头穴丛刺结合环境刺激对缺氧缺血性脑损伤新生大鼠海马MAP-2和p38表达影响的实验研究[J].针灸临床杂志,2011,27(2):63-65,3.基金项目
哈尔滨市科技创新人才研究专项资金项目,编号 （）
2009RFQXS033. （）
