中国药房2011，Vol.22Issue(9)：795-798,4.
咖啡酸对全脑缺血再灌注模型大鼠脑损伤的保护作用研究
Protective Effects of Caffeic Acid on Global Cerebral Ischemia/Reperfusion Injury in Rats
摘要
Abstract
OBJECTIVE: To observe the effect of caffeic acid on global cerebral ischemia/reperfusion injury in rats and its mechanism.METHODS: The rats were randomly divided into the sham operation group (normal saline), model group (normal saline) ,the caffeic acid low dose group, moderate dose group and high dose group (caffeic acid 10, 30, 50 mg·kg-1) with each group of 7 rats.After intraperitoneal injection of corresponding drugs, global cerebral ischemia/reperfusion injury model was established.Morris water maze was used to evaluate the ability of spatial learning and memory function of rat using incubation period as index.HE staining was used to observe histopathological changes of hippocampus neurons and neuron count within view.SOD activity and MDA content were analyzed and NF-κBp65 expression was detected.RESULTS: Compared with model group, caffeic acid remarkably decreased incubation period within 5 d (P＜0.05 or P＜0.01), relieve hippocampal neurons injury and increase neuron count (P＜0.05 or P＜0.01 ).NF-κBp65 expression and MDA content decreased significantly, and SOD activities increased significantly in hippocampus (P＜0.05 or P＜0.01).CONCLUSIONS: Caffeic acid has an obvious protective effect on global cerebral ischemia/reperfusion injury in rats by reducing expression of NF-κBp65 and inhibiting inflammation and oxidative stress of central nervous system.关键词
咖啡酸/全脑缺血再灌注/大鼠/脑损伤/保护作用分类
医药卫生引用本文复制引用
石斌,杨俊卿,姜蓉,罗维楠..咖啡酸对全脑缺血再灌注模型大鼠脑损伤的保护作用研究[J].中国药房,2011,22(9):795-798,4.基金项目
国家自然科学基金资助项目(30672211、81070972) （30672211、81070972）
