中国病理生理杂志2011，Vol.27Issue(4)：643-647,5.DOI:10.3969/j.issn.1000-4718.2011.04.004
丁苯酞对缺氧缺糖条件下血管内皮细胞VEGF和HIF-1α表达的影响
Effects of NBP on expression of VEGF and HIF-1α in HUVECs under the condition of oxygen-glucose deprivation
摘要
Abstract
AIM : To investigate the mechanism that dl - 3 - n - butylphthalide ( NBP) protects cells from the induced by oxygen - glucose deprivation ( OGD) .METHODS : Human umbilical vein endothelial cells ( HUVECs) were exposed to OGD to induce endothelial damage.Endothelial injury was assessed by measuring the changes of chromatin morphology and MTT method.The protein levels of vascular endothelial growth factor ( VECF) and hypoxia inducible factor I alpha ( HIF - 1α) were determined by immunofluorescence and quantitatively analyzed with the software IPP.Western blotting was applied to verify the results.RESULTS : NBP at the concentrations of 0.01 to 100 μmol/L dose - depenclently protected against OGD - induced cell damage.Compared with OGD group, NBP enhanced OGD - induced the expression of VEGF and HIF - 1α , and the difference was statistically significant.The expression of VEGF and HIF - 1α reached to the peak at the time points of 6 h and 8 h after OGD, respectively.CONCLUSION: Under the condition of OGD, NBP enhances the expression of HIF - 1α in HUVECs, subsequently promotes the expression of downstream VEGF, and eventually elevates the survival of the cells.关键词
丁苯酞/血管内皮细胞/缺氧缺糖/血管内皮生长因子/缺氧诱导因子-1分类
医药卫生引用本文复制引用
殷建瑞,张波,谭丽华,傅贤,魏欢,李玲..丁苯酞对缺氧缺糖条件下血管内皮细胞VEGF和HIF-1α表达的影响[J].中国病理生理杂志,2011,27(4):643-647,5.基金项目
广东省科技计划资助项目(No.2008B080703029) （No.2008B080703029）
广东省科技计划资助项目(No.2010B080701008) （No.2010B080701008）
国家自然科学基金资助项目(No.81071069) （No.81071069）
