首页|期刊导航|中华医学杂志（英文版）|Expression of adenosine receptors in human retinal pigment epithelium cells in vitro
中华医学杂志（英文版）2011，Vol.124Issue(8)：1139-1144,6.DOI:10.3760/cma.j.issn.0366-6999.2011.08.004
Expression of adenosine receptors in human retinal pigment epithelium cells in vitro
Expression of adenosine receptors in human retinal pigment epithelium cells in vitro
摘要
Abstract
Background Adenosine receptors (ADORs) have been reported to play a role in experimental myopia. This study aimed to determine the distribution of ADORs in human retinal pigment epithelium (RPE) cells cultured in vitro.Methods Human RPE cells (cell line D407) were cultured in vitro. ADOR mRNA in RPE was detected by reverse transcription polymerase chain reaction. ADOR protein expression in RPE was confirmed by Western blotting analysis of cell lysates. Confocal fluorescence microscopy was used to study the subcellular distribution of ADORs.Results All four subtypes of ADORs mRNA and protein were expressed in human RPE. This was confirmed by Western blotting analysis. The ADOR subtypes were differently distributed within the cells. ADORA1 was expressed in nucleus, perinucleus and cytoplasm of RPE. ADORA2A was concentrated mainly in one side of the perinucleus and cytoplasm of RPE. ADORA2B was strongly expressed in the nucleus, perinucleus and the cytoplasm, and ADORA3 was expressed weakly in the cytoplasm of RPE.Conclusions ADORs are expressed in human RPE. The different distribution at the subcellular level suggests different functions of ADOR subtypes.关键词
retinal pigment epithelium cell/adenosine receptors/cell cultureKey words
retinal pigment epithelium cell/adenosine receptors/cell culture引用本文复制引用
WAN Wen-juan,CUI Dong-mei,YANG Xiao,HU Jian-min,LI Chuan-xu,HU Shou-long,Klaus Trier,ZENG Jun-wen..Expression of adenosine receptors in human retinal pigment epithelium cells in vitro[J].中华医学杂志（英文版）,2011,124(8):1139-1144,6.基金项目
This study was supported by grants from Medical Scientific Research Foundation of Guangdong Province (No.B2009205),Natural Science Foundation of Guangdong Province (No.7301441),and Science and Technology Planning Project of Guangdong Province,China (No.2009B030801169). （No.B2009205）
