中国病理生理杂志2011，Vol.27Issue(4)：648-652,5.DOI:10.3969/j.issn.1000-4718.2011.04.005
EDRV和DIDS对急性缺血再灌注心肌组织活性氧水平的影响
Effects of EDRV and DIDS on reactive oxygen species levels in acute ischemia-reperfusion injured myocardium
摘要
Abstract
AIM : To investigate the effects of chloride channel inhibitor 4,4' - diisothiocyanostilbene - 2,2' disulfonic acid ( DIDS) and free radical scavenger edaravone( EDRV) on the production of reactive oxygen species in acute ischemia - reperfusion injured ( I/RI) myocardium.METHODS : Male Sprague - Dawley rats, subjected to myocardial ischemia for 30 min and reperfusifor for 4 h, were divided into 5 groups: sham group, I/RI group, DIDS group, EDRV group and DIDS + EDRV group.The rats were treated with EDRV ( 10 mg/kg for 5 min) before reperfusion or/and DIDS ( 14 mg/kg,4 mL · kg-1 · h-1 for 2 h) at the beginning of reperfusion by a program - controlled injection micropump .The myocardiac tissues were collected immediately at the end of reperfusion.The levels of ROS, OH · and O-.2 were determined by the methods of spectrofluorophotometry and colorimetry.Myocardial apoptosis was detected by TUNEL method.Serum superoxide dismutase ( SOD) activity and malondialdehyde ( MDA) concentration were measured by colorimetry.RESULTS : Compared with I/RI group, myocardial apoptotic index, the levels of ROS, O-.2 , OH· and MDA were significantly reduced, and SOD activity was significantly increased in DIDS group, EDRV group and DIDS + EDRV group (P ＜0.05) .Compared with DIDS group, the levels of ROS,O-.2 , OH · and MDA were significantly decreased, and SOD activity was significantly increased in EDRV group ( P ＜ 0.05 ) .No statistical difference of myocardial apoptotic index between these two groups was observed ( P ＞ 0.05 ) .Compared with DIDS + EDRV group, myocardial apoptotic index in DIDS group and EDRV group was significantly increased (P＜0.05) , and no significant difference of ROS, O-.2 , OH · , MDA and SOD between the two groups was found ( P ＞ 0.05 ) .CONCLUSION : DIDS and EDRV protect myocardial cells from apoptosis by inhibition of ROS activity.Combinative use of the two reagents has stronger cardioprotectiue effect, suggesting that they have different regulatory pathways.关键词
4,4'-二异硫氰基芪-2,2'-二磺酸/再灌注/活性氧/依达拉奉/心脏分类
医药卫生引用本文复制引用
刘艳霞,刘佳妮,沈明志,李榕,张荣怀,翟雅莉,赵萌,王跃民,王晓明..EDRV和DIDS对急性缺血再灌注心肌组织活性氧水平的影响[J].中国病理生理杂志,2011,27(4):648-652,5.基金项目
国家自然科学基金资助项目 (No.30770847 （）
No.81070127) （）
