中国病理生理杂志2011，Vol.27Issue(4)：769-774,6.DOI:10.3969/j.issn.1000-4718.2011.04.028
尾悬吊模拟失重对雌性大鼠生殖功能的影响
Effects of weightlessness simulated by tail-ventrofixation on reproductive functions in female rats
贾木天 1宋昕宸 2张怡明 1王立祥2
作者信息
- 1. 山东大学医学院药理学研究所,山东,济南,250012
- 2. 山东省实验中学,山东,济南,250001
- 折叠
摘要
Abstract
AIM: To investigate the effects of weightlessness simulated by tail - ventrofixation on reproductive functions in female rats and the underlying mechanism, and to provide some clues to health protection for the space explorers.METHODS: To mimic the weightless state, the rat model of tail - ventrofixation was set up.Seventy - two female Wistar rats were randomly divided into 6 groups: 7 - day, 14 - day and 21 - day tail - ventrofixation groups and the corresponding 3 control groups without tail - ventrofixation.The levels of estradiol ( E2 ), follicle stimulating hormone ( FSH ) and luteinizing hormone ( LH ) in the serum were detected by ELISA.The morphological characters of the ovary were examined under microscope with HE staining.The character of senescence was checked by senescence - specific lipofuscin staining in situ.The RNA of the ovary was extracted to detect the mRNA expression levels of telomerase reverse transeriptase ( TERT),p53, p27,p21 and P16 by qRT-PCR.The protein levels except TERT were also detected by immunohistochemistry.The male Wistar rats cohabited with the female ones to test the quantity and the survival rate of their offspring.RESULTS : Compared with the corresponding control groups, the serum level of E2 decreased in tail - ventrofixation groups ( P ＜0.05 ).However, the levels of FSH and LH increased ( P ＜0.05 ).At the same time, the number of ovarian follicle in tail - ventrofixation groups reduced compared with the corresponding control groups, and the ovary cells showed the characteristics of senescence.The expression of TERT was inhibited in tail - ventrofixation groups compared with the corresponding control groups not only in the level of mRNA but also of the proteins ( P ＜0.05 ).Meanwhile, the expression of p53 , p27 and pl6 increased ( P ＜ 0.05 ).Both the number and the survival rate of offspring rats decreased in tail - ventrofixation groups as compared with the corresponding control groups ( P ＜ 0.01 ).CONCLUSION: The state of simulated weightlessness results in decreased reproductive functions in female rats.Simulated weightlessness induces cell senescence and endocrine dysfunction of hypothalamus - pituitary - ovarian axis.关键词
尾悬吊/失重/卵巢/生殖分类
医药卫生引用本文复制引用
贾木天,宋昕宸,张怡明,王立祥..尾悬吊模拟失重对雌性大鼠生殖功能的影响[J].中国病理生理杂志,2011,27(4):769-774,6.
