中国病理生理杂志2011，Vol.27Issue(4)：775-778,4.DOI:10.3969/j.issn.1000-4718.2011.04.029
尿胞外体亮氨酸氨基肽酶及二肽基肽酶在糖尿病肾病中的变化
Changes of urinary exosomal leucine aminopeptidase and dipeptidyl peptidase 4 in diabetic nephropathy
摘要
Abstract
AIM: To investigate the changes of urinary exosomal enzymes and the correlation with diabetic nephropathy.METHODS: Thirty - four healthy volunteers and 127 patients of type 2 diabetes mellitus ( T2DM ) were included in the study.The healthy volunteers served as control.The patients with T2DM were divided into 3 groups based on their 24 h urinary albumin/creatinine ratio ( UACR ): 50 patients with microalbuminuria in early DN group ( DN1 ), 34 patients with macroalbuminuria in overt DN group ( DN2 ) and 43 patients without albuminuria in DM group.The levels of urine exosomal leucine aminopeptidase( exosome - LAP ) and exosomal dipeptidyl peptidase 4( exosome - DPP4 ) were determined by enzyme - linked immunosorbent assay ( ELISA ).The following methods were used to determine the biochemical parameters: liquid chromatography for glycated hemoglobin ( HbA1c ), chemical modification method for cholesterol ( CH ), Jaffe - kinetic assay for creatinine ( CR ) and urease - GLDH method for blood urea nitrogen ( BUN ).Multiple stepwise linear regressions were used to analyze the relationship of exosome - LAP or exosome - DPP4 with HbA1c, CH, UACR, CR and BUN.RESULTS: The levels of exosome - LAP and exosome - DPP4 in DM, DN1 and DN2 groups were significantly higher than those in control group ( P ＜0.01 ).The exosome - LAP in DN2 group was significantly higher than that in DM group.Correlation analysis showed that the levels of urinary exosome - LAP and exosome - DPP4 were positively correlated with HbA1c, CH, UACR, CR and BUN.Multiple stepwise linear regression analysis showed that CH and UACR were independent determinants for exosome - LAP ( P ＜0.01 ), and UACR and HbAlc were independent determinants for exosome - DPP4 ( P ＜0.01 ).CONCLUSION: Urine exosome - LAP and exosome - DPP4 are correlated with the severity of diabetic nephropathy.These parameters may serve as clinical markers for the diagnosis and prognosis evaluation of diabetic nephropathy.关键词
糖尿病肾病/白蛋白尿/尿胞外体/亮氨酸氨肽酶/二肽基肽酶4分类
医药卫生引用本文复制引用
孙爱丽,胡晓燕,关广聚,邓浩萍,刘元涛,陈诗鸿,刘军莉,邓景惕..尿胞外体亮氨酸氨基肽酶及二肽基肽酶在糖尿病肾病中的变化[J].中国病理生理杂志,2011,27(4):775-778,4.基金项目
山东省自然科学基金资助项目(No.ZR2009CM061) （No.ZR2009CM061）
