中国病理生理杂志2011，Vol.27Issue(4)：787-790,4.DOI:10.3969/j.issn.1000-4718.2011.04.032
全反式维甲酸对人胚肺成纤维细胞增殖及α-SMA表达的影响
Effect of all-trans-retinoic acid on the proliferation and expression of α-SMA in human embryonic lung fibroblasts
摘要
Abstract
AIM: To investigate the effects of all - trans - retinoic acid ( ATRA ) on the proliferation and differentiation of transforming growth factor β1 ( TGF - β1 ) - stimulated human embryonic lung fibroblasts ( HFL - Ⅰ ).METHODS: The HFL - Ⅰ cells were cultured in vitro and were pretreated with ATRA for 3 days at the concentrations of 0.1 μmol/L, 1 μmol/L and 10 μmol/L.The proliferation of HFL - 1 cells was detected by MTT method.The mRNA expression of α - smooth muscle actin( α - SMA ) in HFL - Ⅰ cells stimulated with TGF - β1 for 0 h, 6 h, 12 h, 24 h, 48 h and 72 h was detected by RT - PCR and the protein expression of α - SMA at the time points of 1,3 and 5 days was detected by Western blotting.The mRNA expression of α - SMA in HFL - Ⅰ cells pretreated with different concentrations of ATRA for 24 h was detected the by RT - PCR and the protein expression at time point of 3rd day was detected by Western blotting.RESULTS: Different concentration of ATRA inhibited the proliferation of HFL - Ⅰ in a dose - dependent manner ( P ＜0.05 ).Both mRNA and protein expression of α - SMA in HFL - Ⅰ cells pretreated with TGF - β1 was up - regulated ( P ＜0.05 ).ATRA down - regulated the mRNA and protein expression of α - SMA induced by TGF - β1 in a dose - dependent manner ( P ＜0.05 ).CONCLUSION: ATRA inhibits the proliferation and TGF - β1 - stimulated differentiation in HFL - Ⅰ cells by down - regulating the mRNA and protein expression of α - SMA.关键词
肺纤维化/肌成纤维细胞/转化生长因子β/全反式维甲酸分类
医药卫生引用本文复制引用
李佳鑫,海广范,贾岩龙,夏武,陈永凤,刘巨源..全反式维甲酸对人胚肺成纤维细胞增殖及α-SMA表达的影响[J].中国病理生理杂志,2011,27(4):787-790,4.基金项目
河南省教育厅自然科学研究资助项目(No.2009A310007) （No.2009A310007）
