肿瘤防治研究2011，Vol.38Issue(2)：134-136,140,4.DOI:10.3971/j.issn.1000-8578.2011.02.004
裙带菜多糖对胃癌细胞BGC-823的影响
Effect of Polysaccharides from Undaria Pinnatifida on Gastric Carcinoma BGC-823 Cells
摘要
Abstract
Objective Effect of polysaccharides from the Undaria Pinnatifida on the inhibition of gastric carcinoma cell BGC-823 and its mechanism were studied. Methods After treatment with polysaccharides from the Undaria Pinnatifida at different concentrations (25, 50, 100 μg/ml)for 24 h, 48 h, 72 h, the inhibitory effect on proliferation of BGC-823 cells was analyzed by MTT method. After treatment with polysaccharides from the Undaria Pinnatifida under different concentrations (2. 5, 50, 100 μg/ml) for 48 h, the cell structures were observed by transmission electron microscope. The cell apoptotic ratio of BGC-823 cells was measured with flow cytometry (FCM) and the expression of survivin mRNA was detected by RT-PCR. Results Polysaccharides from the Undaria Pinnatifida inhibited the proliferation of BGC-823 cells significantly in a concentration-depending manner (P＜0. 05).The IC50 was 38. 13μg/ml, after treatment with polysaccharides from the Undaria Pinnatifida in different concentrations for 24 h. The cell apoptosis of BGC-823 cells were induced significantly and have a characteristic structure changes of apoptosis. The apoptotic ratio is 69. 91％ treated with 100 μg/ml of PUP,and the expression level of survivin mRNA were decreased significantly (P＜0. 01 ). Conclusion PUP can inhibit the proliferation, inducing apoptosis of BGC-823 cell. This effect was associated with the down-regulation expression of survivin gene.关键词
胃癌/裙带菜多糖/细胞凋亡/基因表达分类
医药卫生引用本文复制引用
商晓辉,商晓丽,张勇,赵连梅,张志涛,李艳辉..裙带菜多糖对胃癌细胞BGC-823的影响[J].肿瘤防治研究,2011,38(2):134-136,140,4.基金项目
邯郸市科技局2008年指令性课题资助项目(0823108062-2) （0823108062-2）
