中国组织工程研究与临床康复2011，Vol.15Issue(11)：1901-1904,4.DOI:10.3969/j.issn.1673-8225.2011.11.001
重组人胰岛素样生长因子Ⅰ对成骨细胞的影响
Effects of recombinant human insulin-like growth factor on rat osteoblasts
摘要
Abstract
BACKGROUND:Recombinant human insulin-like growth factor (rhIGF- Ⅰ ) is rich in osteoblasts, which has close contact with bone mineral density.OBJECTIVE:To investigate the effects of rhIGF- Ⅰ on proliferation, apoptosis and synthesis of alkaline phosphatase (ALP) of rat osteoblasts in vitro.METHODS:Rat calvaria cells were cultured in medium with or without rhIGF- Ⅰ. The living cell number was assessed by MTT colorimetric assay. Tumor necrosis factor α (TNF-α) was added in the medium to induce apoptosis. The cell cycle and apoptosis were analyzed by flow cytometry after the cells were incubated with medium containing TNF-α or TNF-α plus rhIGF- Ⅰ . The ALP content within osteoblasts was measured by PNPP method.RESULTS AND CONCLUSION:The cell number of osteoblasts exposed to rhIGF- Ⅰ at 102-105 ng/mL significantly increased compared with control group (P ＜0.05). TNF-α (102-105 ng/L) caused a dose-dependent increase of apoptosis (P ＜ 0.05) and decreased the cell number in S phase (P ＜ 0.05). rhIGF- Ⅰ inhibited the inducement of TNF-α for apoptosis. The ALP activity in rhIGF- Ⅰ -treated cells was higher than that in vehicle-treated cells (P ＜ 0.05). IGF- Ⅰ can stimulate proliferation of osteoblasts and inhibit apoptosis induced by TNF-α in vitro, these results suggested that increasing the cell number of osteoblasts might be one of the mechanisms for IGF- Ⅰ to promote bone formation. IGF- Ⅰ can increase ALP synthesis in osteoblasts in vitro, this result suggested that IGF- Ⅰ could probably promote the synthesis of organic matrix and mineralize action of bone.关键词
重组人胰岛素样生长因子Ⅰ/成骨细胞/增殖/凋亡/碱性磷酸酶/肿瘤坏死因子α/骨组织工程分类
基础医学引用本文复制引用
徐萍,张克勤,刘超,陈军建,袁国跃,俞力,钱方方..重组人胰岛素样生长因子Ⅰ对成骨细胞的影响[J].中国组织工程研究与临床康复,2011,15(11):1901-1904,4.基金项目
江苏省自然科学基金(BK2009208),C反应蛋白调控脂联素表达的分子机制研究. （BK2009208）
