中国组织工程研究与临床康复2011，Vol.15Issue(11)：1941-1944,4.DOI:10.3969/j.issn.1673-8225.2011.11.010
低频脉冲超声干预膝骨关节炎模型兔关节软骨及滑膜水通道蛋白3的表达
Effect of low-frequency pulsed ultrasound on expression of articular cartilage and synovial aquaporin 3 in rabbit knee osteoarthritic models
唐进 1黄良库 1彭李华 1李东 1陈世荣1
作者信息
- 1. 重庆医科大学附属第二医院骨科,重庆市,400016
- 折叠
摘要
Abstract
BACKGROUND: Previous studies demonstrated that, low-frequency pulsed ultrasound can promote repair of full-thickness articular cartilage defects, delay the progression of knee osteoarthritis. Aquaporin 3 exists on articular cartilage and synovial membrane, which plays an important role in osteoarthritis.OBJECTIVE: To observe the effect of low-frequency pulsed ultrasound on the expression of articular cartilage and synovial aquaporin 3 in rabbits with knee osteoarthritis.METHODS: Experimental osteoarthritis was replicated in knees of mature New Zealand rabbits by fixing their left back knees straightenedly in plaster cast. The experimental animals received IFPUS therapy, of which the intensity was 2.0 W, and the frequency was 1 MHz. The IFPUS therapy was administered once a day and lasted for 15 minutes every time. For the control group, sham US therapy was administered. The immunohistochemistry was used to detect the expression of articular cartilage and synovial aquaporin 3 at 2, 4 and 8 weeks after therapy.RESULTS AND CONCLUSION: With the modeling time prolonged, the expression of articular cartilage and synovial aquaporin 3 in each group were increased. Compared with the control group, the expression of articular cartilage and synovial aquaporin 3 was lower in the experimental group (P ＜ 0.01). It is suggested that the low-frequency pulsed ultrasound might reduce the expression of aquaporin 3 in articular cartilage and synovial at protein level , relieve joint swelling, reduce the severity of articular cartilage and synovium degeneration, and have a protective effect on cartilage and synovial membrane.关键词
低频脉冲超声/骨关节炎/关节软骨/滑膜/水通道蛋白3分类
医药卫生引用本文复制引用
唐进,黄良库,彭李华,李东,陈世荣..低频脉冲超声干预膝骨关节炎模型兔关节软骨及滑膜水通道蛋白3的表达[J].中国组织工程研究与临床康复,2011,15(11):1941-1944,4.
