中国组织工程研究与临床康复2011，Vol.15Issue(11)：1949-1952,4.DOI:10.3969/j.issn.1673-8225.2011.11.012
亚急性创伤性深静脉血栓形成模型大鼠基质金属蛋白酶3的表达
Expression of matrix metalloproteinase-3 in a rat model of subtraumatic deep vein thrombosis
摘要
Abstract
BACKGROUND: Incidence and mortality of traumatic deep vein thrombosis (TDVT) gradually increased in recent years, which generation mechanism remains unclear. Accordingly, it is necessary to explore inherent rules using gene chip technology.OBJECTIVE: To explore the changes and rules of matrix metalloproteinases in the TDVT.METHODS: Ten SD rats selected from 110 served as normal controls, other 100 rats were prepared subtraumatic TDVT models by beating on bilateral posterior limbs combined with hip spica cast fixation. The femoral vein was incised at immediately, 24, 72,120 and 168 hours after operation. Total RNA was extracted from femoral vein, and the gene expression was detected by Affymetrix 230 2.0 genechips.RESULTS AND CONCLUSION: Expression of matrix metalloproteinase-3 was increased with prolongation of damage duration in the experimental group, suggesting matrix metalloproteinase-3 can be one of the most major factors to influence the biologicalstates of TDVT.关键词
深静脉血栓形成/大鼠/亚急性创伤/基因芯片/基质金属蛋白酶3分类
医药卫生引用本文复制引用
章玉冰,郑燕科,胡继红,李文,姚黎清,赵学凌,陈淼,宋恩,李云健,王辉..亚急性创伤性深静脉血栓形成模型大鼠基质金属蛋白酶3的表达[J].中国组织工程研究与临床康复,2011,15(11):1949-1952,4.基金项目
昆明医学院研究生创新基金项目资助(2009D02). （2009D02）
