中国组织工程研究与临床康复2011，Vol.15Issue(11)：2074-2078,5.DOI:10.3969/j.issn.1673-8225.2011.11.043
创伤性深静脉血栓模型大鼠组织蛋白酶B,C基因对血管内皮细胞的作用
Effects of cathepsin B and cathepsin C gene on vascular endothelial cells in a rat model of traumatic deep venous thrombosis
姚黎清 1宁亚 1赵学凌 1章玉冰 1李宏昆 1李文2
作者信息
- 1. 昆明医学院第一附属医院骨科,云南省昆明市,650032
- 2. 云南省人民医院心内科,云南省昆明市,650032
- 折叠
摘要
Abstract
BACKGROUND: Deep venous thrombosis (DVT) always occurs after orthopedic surgery. At present, clinical diagnosis of DVT has been lack of an effective measuring means for a long time. Cathepsin may be an effective biological marker of DVT. OBJECTIVE: To study the expression change of cathepsin B and cathepsin C in the rat blood cells before and after DVT and to investigate the feasibility of cathepsin B and cathepsin C as candidate molecular markers for early diagnosis of DVT. METHODS: Totally 100 Sprague Dawley rats were randomly divided into normal control group (n=10) and model group (n=90). Rat traumatic deep vein thrombosis models were established by clamping the femoral vein and fixing the bilateral hind limbs. According to observation time points and the different situations of thrombosis, rat models were assigned to three subgroups: pre-thrombosis, intra-thrombosis, and non-thrombosis. Blood RNA of each group was extracted and reverse transcribed into cDNA. The expression of cathepsin B and cathepsin C in blood cells was detected using real-time fluorescence quantitative PCR. RESULTS AND CONCLUSUON: Expression of cathepsin B and cathepsin C in the blood cells was obviously expressed in the intra-thrombosis subgroup. There was no significant difference in cathepsin B and cathepsin C expression between pre-thrombosis, non-thrombosis groups and normal control group. These findings suggest that cathepsin B and cathepsin C are closely related to DVP and they can be used as the candidate molecular markers for early diagnosis of DVT.关键词
深静脉血栓形成/组织蛋白酶/早期诊断/分子标志物/大鼠分类
医药卫生引用本文复制引用
姚黎清,宁亚,赵学凌,章玉冰,李宏昆,李文..创伤性深静脉血栓模型大鼠组织蛋白酶B,C基因对血管内皮细胞的作用[J].中国组织工程研究与临床康复,2011,15(11):2074-2078,5.
