中国组织工程研究与临床康复2011，Vol.15Issue(11)：1973-1976,4.DOI:10.3969/j.issn.1673-8225.2011.11.018
大鼠椎间盘纤维环细胞的培养及鉴定
Culture and identification of rat annulus fibrosus cells
摘要
Abstract
BACKGROUND: There is not a criterion for culture of annulus fibrosus cells, thus, there is deficiency in operability and repetitiveness.OBJECTIVE: To establish the annulus cell lines and to observe their continuouschanges, in order to provide the methods and cell model for studying molecular biology in tissue engineering field.METHODS: The annulus fibrosus cells were isolated with enzyme digestion and grew as monolayer. DMEM containing 15％ fetal calf serum was used in the primary culture, which was replaced by DMEM containing 10％ fetal calf serum after cell passage.Morphology, structure and biological characteristics of cells were detected by an inverted phase contrast microscope. The phenotype was identified with the toluiding blue staining and immunocytochemistry.RESULTS AND CONCLUSION: The disc fibrinous ring cells were cultured in monolayer with the round or fusiformis morphology.The primary cells grew more slowly than the subculture cells. The cells displayed intense toluiding blue metachromasia; the immunohistochemical test revealed that the positive expression of type Ⅰ and Ⅱ collagen on the cells. The experiment successfully culture annulus fibrosus cells in vitro.关键词
生物学特性/椎间盘/纤维环/细胞培养/胶原分类
基础医学引用本文复制引用
张坤木,陈少清,宋红梅,何艳,廖军,洪钰,王诗忠..大鼠椎间盘纤维环细胞的培养及鉴定[J].中国组织工程研究与临床康复,2011,15(11):1973-1976,4.基金项目
课题受国家自然科学基金(C190203),卫生部科学研究基金(WKJ2008-2-48),福建省科技厅国际合作项目(2009I0010)和陈可冀中西医结合发展基金(Ckj2008012)资助. （C190203）
