中国组织工程研究与临床康复2011，Vol.15Issue(11)：2034-2037,4.DOI:10.3969/j.issn.1673-8225.2011.11.033
透骨消痛颗粒干预膝骨性关节炎早期环氧合酶2和诱导型一氧化氮合酶的表达
Expression of cyclooxygenase-2 and inducible nitric oxide synthase in primary knee osteoarthritis interfered by Tougu Xiaotong granules
摘要
Abstract
BACKGROUND: Mornda root, radix paeoniae alba from Hangzhou of China, zhongjiefeng, szechwan lovage rhizome composed of Tougu Xiaotong granule can effectively improve the symptoms of knee osteoarthritis (KOA), but the mechanism of it remains unclear.OBJECTIVE: To observe the effect of Tougu Xiaotong granule on primary knee osteoarthritis cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression.METHODS: A total of 90 New Zealand White rabbits were randomly divided into 6 groups: normal group, model group, Tougu Xiaotong granule low-dosage group, Tougu Xiaotong granule midst-dose group, Tougu Xiaotong granule high-dose group, and Zhuangguguanjie pill group. In addition to the normal group, osteoarthritis model was established by improved Hulth method in the other groups. Tougu Xiaotong granule low-dosage group, Tougu Xiaotong granule midst-dose group, Tougu Xiaotong granule high-dose group, and Zhuangguguanjie pill group was based on modeling treated with containing 5, 10, 20 g Tougu Xiaotong granule or Zhuangguguanjie aqueous solution of 10 g pill at every day, respectively. After 4 weeks, the level of nitrogen monoxidum (NO), NOS, and prostaglandin E2 (PGE2) in rabbit synovial fluid, articular cartilage, iNOS and COX-2 in synovium tissue was detected.RESULTS AND CONCLUSION: In the early stage of osteoarthritis, the level of NO, NOS, PGE2, and the expression of COX-2,iNOS in synovium and cartilage were higher than that in normal group. Tougu Xiaotong granule and Zhuangguguanjie pill can reduce the expression of NO, NOS, PGE2, and expression of COX-2 and iNOS in synovium and cartilage. Tougu Xiaotong granule midst-dose group and high-dose group have the most significant effects. It is indicated that Tougu Xiaotong granule can inhibit degradation of cartilage, release clinical sym ptoms to protect articular cartilage, prevent and cu re osteoarthritis by COX-2 and iNOS expression.关键词
透骨消痛颗粒/骨性关节炎/环氧合酶2/诱导型一氧化氮合酶/骨组织工程分类
基础医学引用本文复制引用
刘伯龄,梁珪清,刘献祥,吴广文,李西海..透骨消痛颗粒干预膝骨性关节炎早期环氧合酶2和诱导型一氧化氮合酶的表达[J].中国组织工程研究与临床康复,2011,15(11):2034-2037,4.基金项目
福建省青年人才项目(编号:2007F3121),课题名称:透骨消痛颗粒干预OA早期COX-2和iNOS炎症途径的实验研究 （编号:2007F3121）
福建省中西医结合老年性疾病重点实验室开放课题?陈可冀中西医结合发展基金项目(编号:CKJ2008057 2008J1004-20),课题名称:OA早期COX-2和iNOS炎症途径及中医药干预机制. （编号:CKJ2008057 2008J1004-20）
