中国组织工程研究与临床康复2011，Vol.15Issue(11)：2038-2041,4.DOI:10.3969/j.issn.1673-8225.2011.11.034
金匮肾气丸对肾阳虚证雄性大鼠生精细胞中凋亡相关蛋白Bcl-2、Bax的影响
Effect of Jingguishenqi pill on expression of apoptosis-related protein Bcl-2 and Bax in male mouse with kidney yang deficiency
摘要
Abstract
BACKGROUND: Kidney-deflciency causes sterility. Jingguishenqi pill is a classical Chinese traditional medicine for treating kidney yang deficiency, but its mechanism remains poorly understood.OBJECTIVE: To explore the effect of Jingguishenqi pill on expression of Bcl-2 and Bax in male mice permatogenic cells with kidney yang deficiency.METHODS: Totally 100 mature male rats were divided into 5 groups randomly: blank control group (normal group), model group,Jingguishenqi pill with low dosage group, middle dosage group, high dosage group. Except the normal group, the others were made into model of kidney-yang deficiency by injecting hydrocortisone. After the model preparation, the mice in the normal and model groups were fed with distilled waters, and those in the Jingguishenqi pill with different dosage groups were fed with the same dose of medicine (0.625, 1.250 and 2.500 g/kg) for 30 days continuously. Then, the weight of the left testis was measured,and the blood level of testosterone, and the sperm density, survival rate were examined. Then the expression of Bcl-2 and Bax in spermatogenic cells were investigated with the immunohistochemistry and image analysis.RESULTS AND CONCLUSION : The weight of left testis, blood level of testosterone, sperm density, sperm survival rate in the model group were decreased than that in the control, Jingguishenqi pill with low dosage group, middle dosage group, high dosage group (P ＜ 0.05). The number of cells of expressing Bcl-2 and the intensity of its expression in the model group were lower than that in other four groups (P ＜ 0.05). The number of cells of expressing Bax and the intensity of its expression in the model group were higher than that in other four groups (P ＜ 0.05). Jingguishenqi pill can promote the expression of Bcl-2 but reduce Bax expression, which exerts a certain therapeutic effect on the kidney-yang deficiency in rats.关键词
金匮肾气丸/凋亡/Bcl-2/Bax/肾阳虚证模型/生精功能障碍分类
基础医学引用本文复制引用
刘贺亮,陈长生,秦军,杨波,秦卫军,张更,袁建林..金匮肾气丸对肾阳虚证雄性大鼠生精细胞中凋亡相关蛋白Bcl-2、Bax的影响[J].中国组织工程研究与临床康复,2011,15(11):2038-2041,4.基金项目
国家自然科学基金(30772683) （30772683）
陕西省科技计划攻关项目(2008K04-02). （2008K04-02）
