中国临床药理学杂志2011，Vol.27Issue(2)：149-151,3.
固相萃取高效液相色谱法测定万古霉素血药浓度
Determination the concentration of vancomycin in serum by solid phase extraction and HPLC
张文娟 1宋新文 1汪洋 1许琼 1刘炘 1张华年 1徐华1
作者信息
- 1. 武汉市儿童医院药学部,武汉,430016
- 折叠
摘要
Abstract
Objective To establish a HPLC method for determination the concentration of vancomycin in serum. Methods The analysis was conducted with Hypersil C18 column (4. 6 mm × 200. 0 mm, 10 μm).Acetonitrile -potassium dihydrogen phosphate (9:91 ) was served as mobile phase, the flow number was 0. 8 mL · min-1, and the detecting wavelengh was at 236 nm. Serum sample was applied to solid phase extraction(SPE) column, and the eluting agent was 10％ acetic acid colonial spirit solution. The eluant was collected and was dried in 65 ℃ water bath with nitrogen gas. Result The linear range of vancomycin in serum was 0. 92 ～ 117. 28 mg · L-1. The recovery rate of low concentration, moderate concentration and high concentration (4. 69,23.45,46. 90 mg · L-1 ) was 100. 92％, 101.48％, 104. 31％, respectively. Both of the intra - day and inter - day RSD were less than 5 ％. Conclusion This method is accurate, sensitive and simple. And it is suitable for determination the concentration of vancomycin in serum and the pharmacokinetic study of vancomycin.关键词
万古霉素/血药浓度/高效液相色谱法分类
医药卫生引用本文复制引用
张文娟,宋新文,汪洋,许琼,刘炘,张华年,徐华..固相萃取高效液相色谱法测定万古霉素血药浓度[J].中国临床药理学杂志,2011,27(2):149-151,3.
