重庆医学2011，Vol.40Issue(14)：1361-1363,1366,封2,5.DOI:10.3969/j.issn.1671-8348.2011.14.005
VGluT1表达增加可能改变缺氧性脑损伤致大鼠痫性发作敏感性
Increased expression of VGluT1 may alterat seizure susceptibility following hypoxic cerebral injury
摘要
Abstract
Objective To explore seizure suscepitibility in rats following hypoxic brain injury and vesicular glutamate transporter subtype 1(VGluT1) and vesicular glutamate transporter subtype 2(VGluT 2)expression.Methods SD male rats were assigned to the control rats(n=35)and the rats exposured to global hypoxia induced by 8％ nitrogen oxygen atmosphere(n=35).To test the seizure threshold , rats from both groups(n = 10) were treated with pentylenetetrazol( PTZ 10mg/kg.5 min) intraperitoneally.To test the number of kindled rats and seizure scales , other rats from both groups(n = 15) were injected intraperitoneally subconvulsive dosage of PTZ(35 mg/kg.2d) for 20 d, successively, and then rats kindled by PTZ in both groups were divided into simple kindling group and post-hypoxic kindling group, respectively. Furthermore, VGluT1 and VGluT2 expressions in the temporal cortex, midbrain , and hippocampal were studied in the rest of both group(n = 10) and the rats kindled by PTZ in both groups by using immunohistochemistry and immunoblotting, respectively.Results The rats with exposure to hypoxia exhibited increased seizure suscepttbility to PTZ.VGluT1 expression within temporal cortex and hippocampus regions in hypoxic group were obviously higher than that in control group.Compared with hypoxia and simple kindling groups, VGluT1 expression was markedly enhanced in posthypoxic kindling group(P＜0.05).There was no significant difference in VGluT2 exression in each groups(P＞0.05).Conclusion The seizure susceptibility in adult rats following hypoxic cerebral is increased , while the increased expression of VGluT1 may lead to hyperexcitability in brain, which may play an important role in the alterations of seizure susceptibility following hypoxic cerebral injury.关键词
脑损伤/癫痫/囊泡膜谷氨酸转运载体-1/囊泡膜谷氨酸转运载体-2引用本文复制引用
陈秀,陈莉芬,吴万福,胡长林..VGluT1表达增加可能改变缺氧性脑损伤致大鼠痫性发作敏感性[J].重庆医学,2011,40(14):1361-1363,1366,封2,5.基金项目
重庆市自然科学基金资助项目(CSTC2008BB5236) （CSTC2008BB5236）
四川省教育厅资助项目(08zc036). （08zc036）
