中国组织工程研究与临床康复2011，Vol.15Issue(12)：2171-2174,4.DOI:10.3969/j.issn.1673-8225.2011.12.020
静电纺丝纳米纤维膜作为骨骼肌组织工程支架材料的细胞相容性
Cytocompatibility of electro-spinning nano-fibrous scaffolds for skeletal muscle tissue engineering
摘要
Abstract
BACKGROUND:Some scholars reported that biodegradable collagen plate or poly L-lactic acid (PLLA), poly glycolic acid (PGA),PLLA/PGA copolymer, serve as scaffold materials of skeletal muscle tissue engineering. But these materials have their own advantages and disadvantages, and still can not fulfill the needs of skeletal muscle tissue engineering.OBJECTIVE:To study the feasibility of the electro-spinning nano-fibrous membrane as scaffolds for skeletal muscle tissue engineering.METHODS:The rat myoblasts at the third passage were cultured with extracts obtaining from different kinds of electro-spinning nano-fibrous membranes (7 groups), with an extractant of F12 media containing 20％ bovine calf serum. The adhesion and growth of myoblasts on the nano-fibrous membrane were examined with MTT and scanning electron microscopy methods.RESULTS AND CONCLUSION:The absorbance value between any two groups has no statistically significant difference compared with control group (P ＞ 0.05), while obvious statistical differences of adhesion rate lay in different groups of biomaterials (P ＜ 0.05). The same result lies in the scanning electron microscopy image. A great quantity of myoblasts adhered to the 70％ PLA + 20％ silk fibroin + 10％ collagen group. These myoblasts are spindle-shaped, regularly arranged, and connecting to adjacent cells. In other groups, the myoblasts are less and irregular, much more like myoblasts in recession. The electro-spinning nano-fibrous membrane has no toxicity and no bad effect on the growth of myoblasts. They adhere well on the membrane, especially on the 70％ PLA + 20％ silk fibroin + 1O％ collagen group.关键词
聚乳酸/蚕丝蛋白/胶原/成肌细胞/静电纺丝技术/纳米纤维膜/组织工程分类
基础医学引用本文复制引用
梁爽,李权,唐休发,冯扬,何等旗..静电纺丝纳米纤维膜作为骨骼肌组织工程支架材料的细胞相容性[J].中国组织工程研究与临床康复,2011,15(12):2171-2174,4.基金项目
教育部博士学科点基金项目(20050610077)和国家自然科学基金资助项目(30872890). （20050610077）
