中国组织工程研究与临床康复2011，Vol.15Issue(12)：2265-2269,5.DOI:10.3969/j.issn.1673-8225.2011.12.043
动物源性骨软骨支架复合骨髓间充质干细胞/软骨细胞修复兔膝关节骨软骨复合缺损
Animal-origin osteochondral scaffold combined with bone marrow mesenchymal stem cells/chondrocytes for repair of composite osteochondral defects in rabbit knee joints
摘要
Abstract
BACKGROUND: Though there were many experiments addressing repairing osteochondral defects before, faulty restoration occurred at coupling interfaces. OBJECTIVE: To investigate the feasibility of repairing of osteochondral composite defects in rabbit knees with animal-origin osteochondral scaffold combined with bone marrow mesenchymal stem cells (BMSCs)/chondrocytes.METHODS: New Zealand white rabbits were randomly divided into the experimental, control and blank groups and prepared for unilateral knee joint osteochondral defects. Animal-origin osteochondral scaffold combined with BMSCs/chondrocytes, animal-origin osteochondral scaffold and no material was implanted to repair the defects in the experimental, control and blank groups, respectively. Healing condition was evaluated by gross observation, hematoxylin-eosin staining, and toluidine blue staining at 4, 8, and 12 weeks after operation. RESULTS AND CONCLUSION: At 12 weeks after operation, gross observation showed the defects were repaired completely without local depression and the regenerated tissues were fused with surrounding tissues in the experimental group. Hematoxylin-eosin staining and toluidine blue staining revealed that there were many new hyaline cartilages in the cartilage defects in which columnar cells were lined well and cartilage lacuna was obviously, also, there were many bony tissues in the bone defects. The regeneration cartilage, the underlying subchondral bone and host bone were coupled completely. The toluidine blue positive rate and histologic scores of the experimental group were superior to those of the control and blank groups (P < 0.05). It is demonstrated that animal-origin osteochondral scaffold combined with BMSCs/chondrocytes is an ideal method to repair defects between cartilage and the underlying subchondral bone.关键词
动物源性/支架/骨髓间充质干细胞/软骨细胞/修复/缺损分类
医药卫生引用本文复制引用
谭文成,查振刚,张嘉晴,郑立恒,梁耀中,夏吉生,黄馨霈,吴昊,林宏生..动物源性骨软骨支架复合骨髓间充质干细胞/软骨细胞修复兔膝关节骨软骨复合缺损[J].中国组织工程研究与临床康复,2011,15(12):2265-2269,5.基金项目
澳门医学科技研究基金项目(031/2009/A),广州市科技计划项目(2008Z1-E411).the Macau Medical Science & Technology Research Fund Project,No.031/2009/A （031/2009/A）
Technological Planning Projects of Guangzhou,No.2008Z1-E411 （）
