中国组织工程研究与临床康复2011，Vol.15Issue(10)：1814-1818,5.DOI:10.3969/j.issn.1673-8225.2011.10.022
骨髓间充质干细胞移植后颈动脉损伤球囊核转录因子κBp65和增殖细胞核抗原的表达
Expressions of nuclear factor κBp65 and proliferating cell nuclear antigen in injured carotid artery balloon after bone marrow mesenchymal stem cells transplantation
摘要
Abstract
BACKGROUND: Inflammatory reaction plays an important role in the incidence of restenosis after percutaneous transluminal coronary intervention. Recent studies found that bone marrow mesenchymal stem cells (BMSCs) have strongly characteristics of immunological regulation. However, BMSCs on the inflammatory reaction in vascular injury is rarely reported.OBJECTIVE: To investigate effects of BMSCs transplantation on expression of nuclear factor KBp65 (NF-κBp65) and proliferating cell nuclear antigen (PCNA) after carotid artery balloon injury of rabbits, and to analyze its correlation with intimal proliferation.METHODS: Thirty-six rabbits were prepared for carotid artery atherosclerotic stenosis models and were randomly divided into the control group and BMSCs transplantation group. BMSCs pre-labled by DAPI was infused into rabbits by the ear vein in the BMSCs transplantation group, and the same amount of PBS solution was infused in the control group. The plasma tumor necrosis factor-α (TNF-α) and interleu kin-6 (IL-6) levels were detected by ELIAS in prior to model preparation and at 7, 14, 28 days after transplantation. The NF-κBp65 and PCNA expressions in vessels tissues were detected by immunohistochemical analysis at 14 days after transplantation, and vascular morphological changes were determined by hematoxylin-eosin staining at 28 days after transplantation.RESULTS AND CONCLUSION: The expression of NF-κBp65 and PCNA in the BMSCs transplantation group decreased significantly compared with control group at 14 days after transplantation (P ＜ 0.05). At 7, 14, and 28 days after transplantation, the plasma TNF-α and IL-6 levels in the BMSCs transplantation group were significantly lower than those in the control group (P ＜0.05). The intimal area, the ratio of the intima/media area and the luminal stenosis ratio were significantly decreased in the BMSCs transplantation group than control group at 28 days after transplantation (P ＜ 0.05). BMSCs is capable to decrease the inflammatory reaction of injured vessels and down-regulate PCNA expression by inhibiting NF-κBp65 in injured vessels, and BMSCs may be good for inhibition of injured vessels intimal proliferation.关键词
颈动脉粥样硬化/骨髓间充质干细胞/细胞移植/炎症/再狭窄分类
基础医学引用本文复制引用
刘志江,石蓓,许官学,赵然尊,沈长银,王冬梅,王正龙..骨髓间充质干细胞移植后颈动脉损伤球囊核转录因子κBp65和增殖细胞核抗原的表达[J].中国组织工程研究与临床康复,2011,15(10):1814-1818,5.基金项目
国家自然科学基金资助课题(NSFC30860100)"心肌梗死后RAMP1基因修饰的MSCs移植对血管成形术后再狭窄的作用研究". （NSFC30860100）
