中国组织工程研究与临床康复2011，Vol.15Issue(10)：1823-1826,4.DOI:10.3969/j.issn.1673-8225.2011.10.024
人胎盘绒毛膜来源间充质干细胞的生物学特性
Biological characteristics of human chorionic-derived mesenchymal stem cells
摘要
Abstract
BACKGROUND: Bone marrow is major sou rce of mesenchymal stem cells (MSCs), but the quantity and quality of MSCs in bone marrow have been shown to be gradually reduced with the increase of age. Therefore, it's very important to find a new source of stem cells.OBJECTIVE: To study a new source of MSCs isolated from human chorionic villous and to study its biological characteristics.METHODS: Umbilical cord and amniotic membrane were isolated from clean placenta; the remaining placenta tissue underwent primary culture and subculture. Tandem repetitive sequence (TRS) analysis was used to detect whether the MSCs obtained from placenta is chorionic villous original. Cell growth pattern was detected by MTT assay. Flow cytometry was applied to analyze cell phenotype and stem cell markers. The different differential medium was used to detect its multi-directional differentiation ability.The immunomodulatory properties of these chorionic-derived cells were evaluated by coculturing the cells with peripheral blood mononuclear cells stimulated by phytohemagglutinin (PHA), and the level of interferon-γ (IFN-γ) was detected by enzyme-labeled immunosorbent assay (ELISA).RESULTS AND CONCLUSION: TRS analysis shows that these chorionic-derived cells are derived from human chorionic. They are adherent cells with typical fibroblast morphology. Flow cytometric analysis reveals that human chorionic-derived cells are consistently positive for MSCs labeled with CD73, CD29, CD44, CD90 and CD105, and negative for the hematopoietic markers CD45, CD11b and CD34. The cells can differentiate into osteoblasts and adipogenesis under different conditioned medium cultured conditions. These results proved that these cells are human chorionic-derived MSCs. The human chorionic-derived MSCs can inhibit peripheral blood mononuclear cell stimulated by PHA to secrete γ-interferon.关键词
人绒毛膜间充质干细胞/生物学特性/免疫调节/诱导分化/表面标记分类
基础医学引用本文复制引用
张睿婷,韩忠朝,韩之波,王涛,孟磊,刘志鹏,李奎,及月茹,杨洲鑫,李扬秋..人胎盘绒毛膜来源间充质干细胞的生物学特性[J].中国组织工程研究与临床康复,2011,15(10):1823-1826,4.基金项目
课题受广东省科技厅省部产学研结合示范基地项目(广东省干细胞产业化基地,项目编号:2010B091101004)支持. （广东省干细胞产业化基地,项目编号:2010B091101004）
