中国兽医科学2011，Vol.41Issue(2)：188-192,5.
传染性喉气管炎病毒WG株糖蛋白gD基因主要抗原区的表达
Expression of major antigenic region of glycoprotein gD gene of infectious laryngotracheitis virus WG strain
摘要
Abstract
To express the gD protein of infectious laryngotracheitis infectious virus(ILTV), a pair of primers was designed to amply gD gene according to the ILTV genome sequence(EU675324) available in GenBank. The PCR amplified gD gene was purified and ligated to pGEM-T Easy vector for sequencing. According to sequencing results and DNAStar software analysis of the antigenicity of gD protein, the fragment encoding 256 aa to 405 aa which is more antigenic was selected for design primers. The fragment of gD gene was cloned into pET-32a vector and transformed into Escherichia coli BL21 (DE3). After induction with IPTG,a recombinant protein with the size of 43.5 ku was expressed and named r-gD. Western-blot analysis showed that the r-gD had strong antigenicity,which provided a foundation for preparation of monoclonal antibodies and the development of diagnostic methods for ILTV.关键词
传染性喉气管炎病毒/糖蛋白D/序列分析/蛋白质免疫印迹分类
农业科技引用本文复制引用
魏秀英,陈洪岩,王云峰,石星明,张晶,王玫,赵妍,胡顺磊,崔红玉,全炎铭,闫帅..传染性喉气管炎病毒WG株糖蛋白gD基因主要抗原区的表达[J].中国兽医科学,2011,41(2):188-192,5.基金项目
国家高技术研究发展计划(863)项目(2003AA213020) （863）
