农业环境科学学报2011，Vol.30Issue(2)：375-382,8.
重金属Cu对堆肥过程中微生物群落代谢和水解酶活性的影响
Effect of Heavy Metal Cu on Microbial Community Metabolic Profiles and Hydrolytic Enzyme Activities in Composting
摘要
Abstract
The objective of the study is to research the potential mechanism of heavy metal Cu on composting in high temperature and static state.An experiment was conducted with mixed materials of pig manure, wheat straw, spent mushroom substrate and microbial inoculants to study the effect of heavy metal Cu on temperature, microbial community metabolic profiles and hydrolytic enzyme during composting.The results indicated that CK(without Cu) treatment achieved the temperature requirement of harmlessness, and the high temperature lasted 5 d (of which more than 55 ℃ maintained 4 d).The high temperature phase(＞50 ℃ ) of L treatment(The concentration of Cu was 100 mg· kg-1) only lasted 4 d, the high temperature phase of H treatment(The concentration of Cu was 500 mg· kg-1) lasted 2 d(of which exceeded 55 ℃ kept 1 d).The treatments of L and H didn't achieve the temperature requirement of the harmlessness.The effect of heavy metal Cu on microbial community metabolic profiles during composting was studied with Biolog method, and combined with cluster analysis and PCA.The results showed that the treatment of L could enhance the metabolic capability of amino acids and polymers, while the treatment of H had inhibition effect on the metabolic capability of polymers and intermediate metabolite.In addition, the L treatment could promote the activity of hydrolytic enzyme, however, the H treatment showed depression effect on them.关键词
Cu/高温堆肥/微生物群落代谢/Biolog方法/聚类分析/PCA分析/水解酶Key words
Cu/ high temperature composting/ metabolism of microbial community/ Biolog method/ cluster analysis/ PCA/ hydrolytic enzyme分类
农业科技引用本文复制引用
郭星亮,陈琳,邵丽,谷洁,高华,秦清军,张卫娟,李海龙,陈胜男,刘江,张洪宾..重金属Cu对堆肥过程中微生物群落代谢和水解酶活性的影响[J].农业环境科学学报,2011,30(2):375-382,8.基金项目
国家自然科学基金(40771109,40871119) （40771109,40871119）
科技部星火计划(2008GA850013,2008GA850007) （2008GA850013,2008GA850007）
农业部948项目(2010-Z20) （2010-Z20）
