实用医学杂志2011，Vol.27Issue(7)：1132-1134,3.DOI:10.3969/j.issn.1006-5725.2011.07.003
HSV-1感染对神经胶质瘤细胞NGF和BDNF表达变化的研究
Study of NGF and BDNF expression in HSV-1 infected human glioma cells
摘要
Abstract
Objective To investigate endogenic nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) expression in herpes simplex type-1-virus (HSV-1) -infected neuroglia cells. Methods U251 human glioma cells were cultured in RPMI-I640 medium and infected with HSV-1 in vitro to estahlish a cell model of viral infection. Establishment of HSV-1-infected U251 cells was identified by HSV-1 gD mRNA expression. NGF and BDNF expression was detected by RT-PCR and Western blotting , respectively, in normal U251 cells and HSV-1-infected U251 cells. Results HSV-1 could efficiently infect U251 cells. NGF and BDNF was identified to he expressed in U251. NGF and BDNF expression reached a maximum at 6 hours post HSV-1 infection, then NGF and BDNF expression decreased in time-dependent manner. Conclusions HSV-1 infection could dysregulate NGF and BDNF expression in the U251 cells.关键词
神经胶质瘤/单纯疱疹病毒1型/神经生长因子/脑源性神经生长因子引用本文复制引用
侯云,王斌,李玲,胡明,辛晓妮,钱冬萌,闫志勇,赵巍,宋旭霞..HSV-1感染对神经胶质瘤细胞NGF和BDNF表达变化的研究[J].实用医学杂志,2011,27(7):1132-1134,3.基金项目
国家自然科学基金项目(编号:30770105,81070501) （编号:30770105,81070501）
"泰山学者"建设工程专项经费资助项目 （）
