实用医学杂志2011，Vol.27Issue(7)：1151-1153,3.DOI:10.3969/j.issn.1006-5725.2011.07.010
不同品系大鼠皮下脂肪源干细胞诱导分化能力的比较
The Comparison of differentiation potency of adipose-derived stem cells from sprague-dawley rats and wistar rats
摘要
Abstract
Objective To investigate the difference of differentiation potency of adipose-derived stem cells (ADSCs) from Sprague-Dawley (SD) rats and Wistar Rats. Methods ADSCs were isolated from the fat pad located in pars inguinalis of 6 SD rats and 6 Wistar rats and cultured in vitro. The morphology of ADSCs was observed using phase-contrast microscopy. ADSCs at generation 4th were cultured under adipogenic and osteogenic condition to conform their differentiation potential. The morphological characteristics of inductive cells were observed using histological staining such as alizarin red for mineralization nodules and oil red O for lipid accumulation during osteogenic and adipogenic induction. Results There was no significant difference in cell morphology of ADSCs between SD rats and Wistar rats. Both of them have the capacity to differentiate toward the adipogenic and osteogenic lineages. Conclusion ADSCs obtained from SD rats show similar cell differentiation potential to those ohtained from Wistar rats. Therefore , these two kinds of ADSCs are hoth favorable choice for stem cell transplantation and tissue engineering.关键词
干细胞/分化/腹股沟脂肪垫/成脂肪诱导/成骨诱导引用本文复制引用
曲戎梅,姚红卫,戴景兴,陈白虹,张艳玲,林来兴妹,原林..不同品系大鼠皮下脂肪源干细胞诱导分化能力的比较[J].实用医学杂志,2011,27(7):1151-1153,3.基金项目
国家重点基础研究计划(973 计划)项目(编号:2007CB512705) （973 计划）
国家自然基金青年面上项目(编号:30801464) （编号:30801464）
