中德临床肿瘤学杂志（英文版）2011，Vol.10Issue(5)：290-292,3.DOI:10.1007/s10330-011-0775-x
脂质体介导质粒DNA细胞转染的优化研究
Optimization on cationic liposome-mediated cell transfection of plasmid DNA
Mingang Ying 1Changhua Zhuo 1Weidong Zang1
作者信息
摘要
Abstract
Objective:The development of gene carriers for efficient gene delivery into cells has attracted growing attention in recent years. The aim of this study was to achieve a better outcome of AAV-293 cells transfection by plasmid DNA.Methods:We studied the optimal condition for higher efficiency of cationic lipid-mediated cell transfection. Four experimental groups were set. Plasmid DNA and liposome were mixed in each groups at different ratios (μg :μL), 1:2.5,1:3.5,1:4.0 and 1:5.0, respectively. LacZ gene functioned as reporter gene, measuring the transfection efficiency of the four groups using the method of X-gal staining. Results:When the ratio was 1:3.5, the cell transfection rate was the highest. While the ratio of 1:2.5recommended by product manual achieve the lowest transfection rate. Their difference had statistical significance. Conclusion:In order to obtain a higher transfection efficiency, optimization on conditions of the ratio of plasmid DNA to liposome is necessary in cell transfection.关键词
cell transfection/cationic lipid/plasmid DNA/cell culture/transfection efficiencyKey words
cell transfection/cationic lipid/plasmid DNA/cell culture/transfection efficiency引用本文复制引用
Mingang Ying,Changhua Zhuo,Weidong Zang..脂质体介导质粒DNA细胞转染的优化研究[J].中德临床肿瘤学杂志（英文版）,2011,10(5):290-292,3.
