中国兽医科学2011，Vol.41Issue(2)：138-142,5.
鲫鱼ghrelin基因的克隆和生物信息学分析
Cloning and bioinformatics analysis of ghrelin gene in Carassius auratus
摘要
Abstract
The biological function of ghrelin gene in crucian carp was explored to provide a basis to study effect of the gene on appetite of crucian.The ghrelin gene was amplified from total RNA extracted from the crucian carp intestine by RT-PCR and sequenced and the structure and function of the protein encoded by the gene were investigated.The function of the ghrelin gene-encoded protein was predicted by the bioinformatics software and the phylogenetic tree of ghrelin gene was constructed by CLUSTALW software.In result,the amplified crucian carp ghrelin protein gene was 490 bp in length and submitted to GenBank(GenBank accession number: HM567312).Bioinformatics analysis showed that the gene contained the complete coding sequence of ghrelin protein,which was 312 bp in size, encoded 103 amino acids.The crucian carp ghrelin was 11 519.48 u in molecular weight,5.20 in isoelectric point,about 1.20 hours in half-life,and 31.29 in instability index.The mean of hydropathicity of ghrelin protein was 0.727, while the hydrophobicity was between -2.900 and 2.856.The protein had one signal peptide between site 1 and 26,with 44 α-helix,8 β-sheet and 43 coils.The phylogenetic tree showed that the evolution distance of crucian carp ghrelin gene was the most homogeneous to goldfish ghrelin gene.关键词
鲫鱼/ghrelin基因/克隆/生物信息学分析分类
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周朝伟,林方军,刘遥鹏,胡海平,刘晓凤,张兴栋,刘涛,李洋,李志琼..鲫鱼ghrelin基因的克隆和生物信息学分析[J].中国兽医科学,2011,41(2):138-142,5.基金项目
四川农业大学双支计划项目(01570702) （01570702）
四川农业大学科研兴趣培养项目(04050637) （04050637）
