军医进修学院学报2011，Vol.32Issue(3)：280-281,284,3.DOI:CNKI:11-3275/R.20101119.1541.017
两种材料复合rhBMP-2诱导大鼠皮下异位成骨的比较研究
Subcutaneous ectopic osteogenesis induced by porous calcium phosphate cement and gelatin sponge as the carrier of recombinant bone morphogenetic protein-2 in rats:A comparative study
李想 1董纪元 1彭江 1汪爱媛 1睢翔 1赵斌 1刘道宏1
作者信息
摘要
Abstract
Objective To analyze the difference in subcutaneous ectopic osteogenesis induced by porous calcium phosphate cement (CPC) and gelatin sponge as a carrier of recombinant bone morphogenetic protein-2 (rhBMP-2). Methods Thirty Sprague Dawley rats with an average body weight of 200g were divided into groups A-D. CPC+rhBMP-2, CPC, gelatin sponge+rhBMP-2, and gelatin sponge were implanted into the rats after anesthesia. Ten rats were killed 2, 4 and 8 weeks after they were fed under sterile environment. Bone tissue samples were collected from the implantation sites. Tissue mineral density (TMD) and trabecular thickness were detected with micro-CT scanner and analyzed with SPSS 1 OX) statistical software. Bone tissue was fixed in 4% paraformaldehyde for 2 days, embedded in paraffin, and cut into sections. The sections were stained with H&E to observe their histological change. Results The tissue mineral density and trabecular thickness of the samples with rhBMP-2 were higher in two experimental groups 2,4 and 8 weeks after implantation, which increased with the prolongation of time (P<0.05). Conclusion Porous CPC can be used as a carrier of rhBMP-2 for osteogenesis.关键词
骨形态发生蛋白/骨生成/大鼠/异位成骨Key words
rhBMP-2/ Osteogenesis/ Rats/ Ectopic Osteogenesis分类
医药卫生引用本文复制引用
李想,董纪元,彭江,汪爱媛,睢翔,赵斌,刘道宏..两种材料复合rhBMP-2诱导大鼠皮下异位成骨的比较研究[J].军医进修学院学报,2011,32(3):280-281,284,3.
