南京医科大学学报（自然科学版）2011，Vol.31Issue(2)：170-174,5.
雷公藤甲素对人肝癌细胞株HepG2体内外作用的研究
The antitumor effect of triptolide on HepG2 cells in vitro and in vivo
摘要
Abstract
Objective: To study the effect of triptolide on human hepatocelluar carcinoma HepG2 cells proliferation and apoptosis in vitro and in vivo, as well as its possible mechanisms.Methods:HepG2 cells were cultured in triptolide media with different concentrations.Cell viability was assessed by MTT assay.Cell apoptosis was observed by flow cytometry (FCM).The heat shock protein 70(HSP70) was assayed by Western blotting and RT-PCR methods.Caspase-3 activity was examined by caspase-3 colorimetric assay.In a xenograft tumor model, the control group and experimental group were treated with 0.9％ NaCl solution and triptolide(0.4mg/kg, q.d.), respectively.Three weeks after treatment, the influence of triptolide on the growth of hepatocellular carcinoma was observed.Results:Triptolide treatment resulted in dose- and time-dependent proliferation and apoptosis of HepG2 cells(P ＜ 0.05).Tfiptolide decreased HSP70 mRNA and protein levels in a dose-dependent pattern, whereas caspase-3 activity was significantly increased in a dose-dependent manner.Moreover, Mice receiving triptolide therapy had significanfiy smaller tumors than controls (P ＜ 0.05).Conclusion:Triptolide causes human bepatocelluar carcinoma HepG2 cells death in vitro and in vivo by inducing apoptosis and its mechanism of action might be mediated via the inhibition of HSP70 and up-regulation of caspase-3 activity.关键词
雷公藤甲素/HepG2细胞/热休克蛋白70/caspase-3Key words
triptolide/HepG-2 cell/heat shock protein 70/ caspase-3分类
医药卫生引用本文复制引用
尹亮,王亮,蒋维维,孔连宝..雷公藤甲素对人肝癌细胞株HepG2体内外作用的研究[J].南京医科大学学报（自然科学版）,2011,31(2):170-174,5.基金项目
江苏省"六大人才高峰"(第五批高层次人才项目) （第五批高层次人才项目）
