南京医科大学学报（自然科学版）2011，Vol.31Issue(2)：179-184,6.
细胞凋亡在人乳腺癌细胞拟态血管形成中的作用
The involvement of apoptosis in vasculogenic mimicry formation of human breast cancer cells
摘要
Abstract
Objective: To investigate the effect of cell apoptosis on the vasculngenic mimicry in human breast cancer cells(MDAMB-231). Methods:The tube formation ability was assessed using a matrigel basement membrane model, and the structures of the tubular networks were observed under the inverted microscope. Apoptosis cells were stained with Annexin-FITC and PI,and then quantified by fluorescent microscope. Using the broad range caspase inhibitor Z-VAD-FMK and the caspase-3 inhibitor Ac-DEVDCHO,we further explored the influence of cell apoptosis on forming capillary like structures. Results: MDA-MB-231 cells linked to each other to form wreaths and network structures after 24 h based on three dimensional culture. Capillary-like structures of MDAMB-231 cells were stained positively with PAS. Apoptosis occurred before capillary-like structure formation but not after capillary-like structures had assembled. Both Z-VAD-FMK and Ac-DEVD-CHO blocked capillary-like structure formation. Conclusion:Breast cancer cells can adopt a capillary-like structure and engage in vasculogenic mimicry. Capillary-like structure formation requires apoptotic cell death through activation of a caspase-dependent mechanism.关键词
细胞凋亡/人乳腺癌细胞(MDA-MB-231)/拟态血管Key words
apoptosis/human breast cancer cells(MDA-MB-231)/vasculogenic mimicry分类
医药卫生引用本文复制引用
李卫星,孙重期,杜军..细胞凋亡在人乳腺癌细胞拟态血管形成中的作用[J].南京医科大学学报（自然科学版）,2011,31(2):179-184,6.基金项目
江苏省高校自然科学研究计划(07KJD310138) （07KJD310138）
南京医科大学基础医学院青年教师培养基金(2010) （2010）
江苏省卫生职业技术教育课题(J200501) （J200501）
江苏省高等学校大学生实践创新训练计划 （）
