细胞外信号调节激酶对氧糖剥夺后大鼠海马神经元的作用研究OA北大核心CSCDCSTPCD
Effect of extracellular signal regulated kinase on cultured hippocampal neurons of rats with oxygen-g1ucose deprivation
目的:研究细胞外信号调节激酶对氧糖剥夺(oxygen-glucose deprivation,OGD)后大鼠海马神经元的作用.方法:建立培养乳鼠海马神经元OGD模型,并分为正常对照组、OGD组、PD 98059 10 μmol/L、30 μmol/L组.流式细胞仪Annexin V/PI双染色法检测神经元细胞凋亡率,Western blot法检测ERKl/2、pERKl/2的表达.结果:与正常对照组相比,OGD组神经元的凋亡率升高(P＜0.01)…查看全部>>
Objective: To investigate the effect of excelluar signal-regulated kinase (ERK) on rat hippocampal neurons with oxygen-glucose deprivation (OGD). Methods:Cultured hippocampal neurons were designed into four groups:normal group,OGD group,PD98059 10 μmol/L and 30 μmoL/L groups. The apoptosis of neurons was determined by flow cytometry, and Western blot was used to evaluate protein expression of ERKI/2 and pERKI/2. Results:Compared with normal control group, ne…查看全部>>
华烨;丁新生;孔岳南
南京医科大学附属无锡二院神经内科,江苏,无锡,214002南京医科大学第一附属医院神经内科,江苏,南京,210029南京医科大学附属无锡二院神经内科,江苏,无锡,214002
医药卫生
细胞外信号调节激酶氧糖剥夺海马神经元凋亡
excelluar signal-regulated kinase oxygen-glucose deprivation hippocampal neuronapoptosis
《南京医科大学学报（自然科学版）》 2011 (2)
185-189,5
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