南京医科大学学报（自然科学版）2011，Vol.31Issue(2)：185-189,5.
细胞外信号调节激酶对氧糖剥夺后大鼠海马神经元的作用研究
Effect of extracellular signal regulated kinase on cultured hippocampal neurons of rats with oxygen-g1ucose deprivation
华烨 1丁新生 2孔岳南1
作者信息
- 1. 南京医科大学附属无锡二院神经内科,江苏,无锡,214002
- 2. 南京医科大学第一附属医院神经内科,江苏,南京,210029
- 折叠
摘要
Abstract
Objective: To investigate the effect of excelluar signal-regulated kinase (ERK) on rat hippocampal neurons with oxygen-glucose deprivation (OGD). Methods:Cultured hippocampal neurons were designed into four groups:normal group,OGD group,PD98059 10 μmol/L and 30 μmoL/L groups. The apoptosis of neurons was determined by flow cytometry, and Western blot was used to evaluate protein expression of ERKI/2 and pERKI/2. Results:Compared with normal control group, neuron apoptosis increased while pERK1/2 protein expression decreased obviously in OGD group (both P ＜ 0.01). Neuron apoptosis increased and pERK1/2 protein expression decreased more significantly in PD98059 groups compared with OGD group (both P ＜ 0.01 ). Changes in 30 μmol/L PD98059 group were more significant than 10 μmol/L PD98059 group (P ＜ 0.01 ). Each group showed no significant change in ERK expression (P ＞ 0.05). Conclusion:ERK may be involved in neuronal apoptosis after OGD, and inhibition of ERK pathway can promote neuronal apoptosis.关键词
细胞外信号调节激酶/氧糖剥夺/海马神经元/凋亡Key words
excelluar signal-regulated kinase/ oxygen-glucose deprivation/ hippocampal neuron/apoptosis分类
医药卫生引用本文复制引用
华烨,丁新生,孔岳南..细胞外信号调节激酶对氧糖剥夺后大鼠海马神经元的作用研究[J].南京医科大学学报（自然科学版）,2011,31(2):185-189,5.
