畜牧兽医学报2011，Vol.42Issue(2)：284-288,5.
藏系绵羊KAP3.2基因多态性及其对部分经济性状的影响
Polymorphism of KAP3.2 Gene and Its Effect on Partial Economic Traits in Tibetan Sheep
摘要
Abstract
PCR-SSCP and DNA sequencing techniques were used to analyze the polymorphism of partial sequence of KAP3.2 gene in 254 Tibetan sheep including three types (Plateau, Oula and Qiaoke). Based on the data, the correlation between the polymorphisms and production traits (body weight, wool length and wool yield) was studied using least square linear model. The results showed that three genotypes (AA, AB and BB) were detected at KAP3. 2 gene locus. Allele A was predominant in Plateau type, and allele B was predominant in Oula and Qiaoke types.Frequencies of genotype deviated from Hardy-Weinberg equilibrium in three types. The polymorphism was medlum in Plateau and Oula types, while low in Qiaoke type. There was one mutation (C to T) at 271 bp on KAP3. 2 gene. For the body weight, there was no significant difference among various genotypes in three types of Tibetan sheep. For the wool length, individuals withgenotype AA was significantly higher (P＜0. 05, P＜0. 01) than those with BB genotype, and there was no significant difference between AA and AB genotypes in three type9 of Tibetan sheep. For the wool yield, individuals with AA genotype was significantly higher(P＜0. 05) than those with AB and BB genotypes in three types of Tibetan sheep. The results indicated that the KAP3. 2 could be regarded as marker for wool length and wool yield traits in Tibetan sheep.关键词
藏系绵羊/PCR-SSCP/KAP3.2基因/分子标记分类
农业科技引用本文复制引用
王志有,陈玉林,徐秋良,马忠仁,祁全青..藏系绵羊KAP3.2基因多态性及其对部分经济性状的影响[J].畜牧兽医学报,2011,42(2):284-288,5.基金项目
农业公益性行业科研专项"毛用羊现代产业技术研究与示范" （）
