中国组织工程研究与临床康复2011，Vol.15Issue(18)：3288-3292,5.DOI:10.3969/j.issn.1673-8225.2011.18.015
异位气管移植模型中上皮细胞的增殖与凋亡
Epithelial proliferation and apoptosis in heterotopic tracheal transplantation model
王林毛 1吴凯 2高文 3陈昶 2鲍方 2郑卉2
作者信息
- 1. 苏州大学医学院,江苏省苏州市,215000
- 2. 同济大学医学院附属上海市肺科医院胸外科,上海市200433
- 3. 山东省沂源县人民医院普外科,山东省淄博市,256100
- 折叠
摘要
Abstract
BACKGROUND:The process of epithelial cells in heterotopic trachea transplantation remains unclear. The studies demonstrated that epithelial proliferation is accompanied by irreversible cell death, and airway epithelial regeneration and death occurred before airway occlusion transplantation .OBJECTIVE:To observe epithelial proliferation and apoptosis in the process of heterotopic mouse trachea transplantation.METHODS:The allogeneic BALB/c mouse trachea was implanted subcutaneously in the nape of BALB/c mouse. Ciclosporin A (CsA) was not injected after transplantation. The allogeneic BALB/c mouse trachea was implanted subcutaneously in the nape of C57BL/6 mouse. One group was not injected with CsA, the other group was the whole course injected with CsA in abdominal cavity. At 3, 7, 14, 21, 30 days after transplantation, the integrity of airway epithelium, submucosal infiltration of inflammatory cells and fibrous tissue proliferation were evaluated by hematoxylin-eosin staining. BrdU labelling index and TUNEL positive cells in trachea transplantation were determined.RESULTS AND CONCLUSION:At 3 days after receptor BALB/c mouse transplantation, airway epithelial cells suffered from minor injury and loss. At 7-21 days after transplantation, airway epithelial cells recovered normal gradually. Airway epithelium approached to normal epithelium at 30 days after transplantation. The extent of BrdU labelling index was 3％-5％. The number of TUNEL positive cells was basically unchanged. At 3 days after receptor C57BL/6 mouse transplantation, airway epithelial cells suffered from minor injury. The shedding and necrosis were existed in airway epithelium, at 721 days after transplantation; fibrous proliferation was appeared and became more serious gradually. At 30 days after transplantation, epithelial cells completely disappeared, lumens were filled by fibrous tissue, closed to occlusion. The peak of BrdU labelling index was at 14 days after transplantation. The group of not injected with CsA was higher than group of injected with CsA in BrdU labelling indexes (P=0.000).At 21 and 30 days after transplantation, the BrdU labelling index was significantly reduced. The peak of UNEL positive cells in group of not injected with CsA was at 21 days after transplantation, the peak of UNEL positive cells group of injected with CsA was at 14 days after transplantation. It is indicated that epithelial proliferation in heterotopic trachea transplantation was accompanied by irreversible cell death. CsA could reduce the incidence of bronchiolitis obliterans, but did not prevent its occurrence.关键词
气管移植/同种异体移植/闭塞性气道疾病/同系移植/环孢素A/器官移植分类
医药卫生引用本文复制引用
王林毛,吴凯,高文,陈昶,鲍方,郑卉..异位气管移植模型中上皮细胞的增殖与凋亡[J].中国组织工程研究与临床康复,2011,15(18):3288-3292,5.
