中国组织工程研究与临床康复2011，Vol.15Issue(18)：3293-3297,5.DOI:10.3969/j.issn.1673-8225.2011.18.016
深低温冻储同种异体气管移植并骨髓间充质干细胞移植后气管上皮细胞血管内皮生长因子的表达
Effect of bone marrow derived mesenchymal stem cells transplantation combined with cryopreservation on trachea allograft epithelium vascular endothelial growth factor levels
摘要
Abstract
BACKGROUND:Cryopreserved tissues can maintain their vitality and integrity, and reduce the antigenicity.OBJECTIVE:Using intravenous transplantation of bone marrow derived mesenchymal stem cells (BMSCs) combined with cryopreserved allograft, to investigate the role of BMSCs on enhancing the implant su rvival and revascularization by measuring vascular endothelial growth factor (VEGF) levels.METHODS:After transplanted with cryopreserved 2 weeks and 6 weeks allocraft, PKH-26 labeled 3-5 passage BMSCs were injected into the recipient rats via the tail vein. Rats in the control groups were injected with the same amount of phosphate buffer solution (PBS). We observed the histology of the transplanted trachea and measured the VEGF protein levels in the epithelium to evaluate the role of BMSCs transplantation on the epithelium revascularization.RESULTS AND CONCLUSION:Rats from BMSCs injection group survived a long period. Histological observation showed that the tracheal lumen was covered by psudo-striated ciliated columnar epithelium. VEGF protein levels in the BMSCs injection group were higher than PBS group. BMSCs may exert their reparation function via enhancing epithelium VEGF expression and revascularization.关键词
气管移植/骨髓间充质干细胞/同种异体/深低温冻储/血管内皮生长因子分类
临床医学引用本文复制引用
韩云,纪子钊,蓝妮,庞超见,佟晓杰,石文君..深低温冻储同种异体气管移植并骨髓间充质干细胞移植后气管上皮细胞血管内皮生长因子的表达[J].中国组织工程研究与临床康复,2011,15(18):3293-3297,5.基金项目
本课题受辽宁省教育厅科学技术研究项目(L2010606)资助. （L2010606）
