中国组织工程研究与临床康复2011，Vol.15Issue(18)：3306-3310,5.DOI:10.3969/j.issn.1673-8225.2011.18.019
未成熟树突状细胞表面CD1d分子在体外移植免疫中的作用
Contribution of mouse CD1d to immune toleration induced by immature dendritic cells in vitro
马朝晖 1韩勇 2姚家麟 3姚志发3
作者信息
- 1. 解放军第二一一医院心脏外科,黑龙江省哈尔滨市150080
- 2. 解放军第四军医大学附属唐都医院,陕西省西安市710038
- 3. 哈尔滨医科大学附属第二临床医学院,黑龙江省哈尔滨市150080
- 折叠
摘要
Abstract
BACKGROUND: Dendritic cells (DCs) possess two-way immune regulation ability: mature DCs (mDCs) can activate the immune response, and immature DCs (imDCs) tend to induce immune tolerance.OBJECTIVE: To discuss the role of CD1d in immune toleration induced by imDCs after transplantation and the mechanism of cytokine played in this process.METHODS: imDCs of BALB/c mouse transfected by viral interleukin-10 were stimulated with GM-CSF, LPS in vitro, and separated into three groups after anti-CD1d intervention. The expression of cytokine and phenotypes of the cells were observed. 1st MLC and 2nd MLC were performed in different experimental conditions to observe the immunity of CD1d with heterogeneity T lymphocyte existing. Proliferation of T lymphocyte and the expression of cytokine were observed.RESULTS AND CONCLUSION: Proliferation of heterogeneity T lymphocyte induced by imDC was suppressed by anti-CD1d intervention. The immune function of heterogeneity T lymphocyte was deficient after sensitized by imDCs with anti-CD1d intervention. Anti-CD1d intervention would affect the functional matu rity of imDCs in case the heterogeneity T lymphocyte existed,in that it could suppress the secretion of Th1 type cytokine interleukin-12, however enhance the secretion of Th2 type cytokine interleukin-10.关键词
抗原提呈/CD1d分子/细胞因子/树突状细胞/免疫耐受分类
临床医学引用本文复制引用
马朝晖,韩勇,姚家麟,姚志发..未成熟树突状细胞表面CD1d分子在体外移植免疫中的作用[J].中国组织工程研究与临床康复,2011,15(18):3306-3310,5.
