中国组织工程研究与临床康复2011，Vol.15Issue(18)：3357-3360,4.DOI:10.3969/j.issn.1673-8225.2011.18.031
大鼠热休克蛋白72基因真核表达载体构建及在COS7细胞中的表达
Construction of eukaryotic expression vector carrying rat heat shock protein 72 gene and its expression in COS7 cells
摘要
Abstract
BACKGROUND: In vitro cloning and expression of heat shock proteins (HSP), especially HSP72, is important in the study of ischemia-reperfusion injuries.OBJECTIVE: To construct eukaryotic expression vector carrying HSP72 and study its transient expression in COS7 cells, and to establish the foundation for further research of the immune function of HSP72.METHODS: HSP72 cDNA was amplified from hepatocytes of BABL/C mice by RT-PCR, and correctly inserted into corresponding sites of eukaryotic expression vector pcDNA3.1 (+)after restriction endonuclease digestion, and the recombinant plasmid was transfected into COS7 cells and gene expression was determined by RT-PCR.RESULTS AND CONCLUSION: The gene fragment inserted into the vector pcDNA3.1 (+)was confirmed by nucleotide sequencing, and the recombinant plasmid mRNA was successfully expressed in COS7 cells.关键词
热休克蛋白72/基因表达/COS7细胞/转录/鉴定分类
基础医学引用本文复制引用
晏春根,黄丹文,任光圆,朱冬芳..大鼠热休克蛋白72基因真核表达载体构建及在COS7细胞中的表达[J].中国组织工程研究与临床康复,2011,15(18):3357-3360,4.基金项目
浙江省自然科学基金(Y2100961) （Y2100961）
浙江省科技计划项目(2009C33129). （2009C33129）
