西北国防医学杂志2011，Vol.32Issue(2)：97-99,3.
子宫内膜异位症异位内膜细胞原代培养方法的改良
Improvement of primary culture methods for human ectopic endometrial cells from patients with endometriosis
彭冬先 1何援利 1丘立文2
作者信息
- 1. 南方医科大学珠江医院,妇产科,广东,广州,510282
- 2. 南方医科大学珠江医院,临床医学基础研究所,广东,广州,510282
- 折叠
摘要
Abstract
Objective:To explore a convenient method for primary culture of ectopic endometrial cells from patients with endometriosis.Methods: The ectopic endometrial cells from ten patients with endometriosis were isolated and purified by trypsin digestion and pasted wall purification.The cellular morphology was observed by optical microscope.Endometrial epithelial cell (EEC) and endometrial stromal cell (ESC) were identified by immunocytochemistry.Results:The cell culture was successfully established in 8 of 10 cases of ectopic endometrium.Both EEC and ESC were existed in the culture and stained positively by cytokeratin and vimentin monoclonal antibody,respectively.The life span of EEC and ESC can last 5 weeks and 14 weeks respectively.Conclusion:The ectopic endometrial cells from patients with endometriosis were successfully cultured in vitro.The improved primary culture method for ectopic endometrial cells is convenient and of high performance.关键词
子宫内膜异位症/异位内膜细胞/原代培养分类
医药卫生引用本文复制引用
彭冬先,何援利,丘立文..子宫内膜异位症异位内膜细胞原代培养方法的改良[J].西北国防医学杂志,2011,32(2):97-99,3.
