安徽医科大学学报2011，Vol.46Issue(6)：509-514,6.
MCMV感染同种异型皮肤移植小鼠间质性肺炎模型的建立
A model of interstitial pneumonitis by murine cytomegalovirus infection in mice with allogenic skin transplantation
摘要
Abstract
Objective To establish a mouse model of interstitial pneumonitis with acute murine cytomegalovirus ( MCMV ) after allogeneic skin transplantation. Methods ① 25 of C57BL/6 donor mice and 100 0f BALB/c recipient mice were underwent back skin graft. Cyclosporine A ( CsA,12 mg/kg ) was subsequently given for 14 days.(② All recipient animals were randomly divided into five groups ( n =20 ), four experimental groups were infected intranasally with MCMV infected mouse embryonic fibroblasts ( MEF ) at different concentrations ( 102 ,103,104 and 105 PFU ) in a volume of 30 μl, and the control group was inoculated with 30 μl of MEF cell suspension.After infection days 5,9,14 and 21, mice were sacrificed and lung samples were collected for HE staining, transmission electron microscope( TEM ), PCR/RT-PCR. in situ hybridization, and immunohistochemistry. Results Focal pathological abnormality in the lung was observed in the mice with high dose of virus ( 104 and 105 PFU ).Herpes virus particles were found by TEM in the epithelial of the lung tissue. MCMV DNA was detected by PCR for immediate early( IE ) genes and late genes glycoprotein B( gB ) , and it was localized in lung cells by in situ hybridization. Viral mRNA for IE and gB were also present suggesting lytic infection in the lung tissue. MCMV protein was revealed in the interstitial lung epithelial cells by immunohistochemistry. Clinical manifestations were only observed in the group with 105 PFU in 21 days after infection. Conclusion The study demonstrates that a mouse model of interstitial pneumonitis is successfully established after allogeneic mouse skin transplantation.关键词
鼠巨细胞病毒属/疾病模型,动物分类
医药卫生引用本文复制引用
倪德群,赵纪强,张玮,甘霖,陈敬贤,王明丽..MCMV感染同种异型皮肤移植小鼠间质性肺炎模型的建立[J].安徽医科大学学报,2011,46(6):509-514,6.基金项目
国家自然科学基金(编号:30872253) （编号:30872253）
安徽省科技攻关项目(编号:08010302179) （编号:08010302179）
