广东医学2011，Vol.32Issue(10)：1224-1226,3.
高浓度葡萄糖对培养兔角膜上皮细胞增殖活性的影响
Effects of high glucose on the proliferation activity of cultured rabbit corneal epithelial cells
摘要
Abstract
Objective To study the effects of high glucose on proliferation activity of cultured rabbit corneal epithelial cells in vitro, and to establish culture model of diabetic rabbit corneal epithelial cells. Methods Rabbit corneal epithelia cells were cultured in glucose concentrations at 25 , 40 and 60 mmol/L, for 24 , 48 and 72 hours apiece according to factorial design. SunBioTM Am - Blue cells proliferation and activity assay kit was applied to detect proliferation activity of cultured rabbit corneal epithelial cell in vitro; and microplate reader was used for assessing the OD values. which was the evaluation index of proliferation activity of cell. Cell morphology was observed by inverted microscope. Experiment was repeated three times. Results Significant inhibitation was observed in cell proliferation of cells cultured with high glucose ( F = 276. 318, P ＜ 0. 01 ), with significant differences among cell with different culture time ( F = 38. 727 , P ＜ 0. 01 ).Significant interaction was observed between glucose concentration and culture time on cell proliferation ( F = 2. 687 , P ＜0. 05 ). Conclusion High glucose inhibits the proliferative activity of cultured rabbit corneal epithelial cells in vitro in a time - dependent manner.关键词
高浓度葡萄糖/角膜上皮细胞/增殖活性/兔Key words
high glucose/ corneal epithelial cells/ proliferation activity/ rabbit引用本文复制引用
宋子宣,陈剑,王彦平,阮余霞,陈建苏,吴静,唐光霞,高松哲..高浓度葡萄糖对培养兔角膜上皮细胞增殖活性的影响[J].广东医学,2011,32(10):1224-1226,3.基金项目
国家自然科学基金资助项目(编号:30872808) （编号:30872808）
